伏马菌素产生菌的分离及其产毒检测

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 691-693.

伏马菌素产生菌的分离及其产毒检测

侯红漫，张笑，张昊，陈莉，刘阳

大连轻工业学院生物与食品工程学院；

出版日期:2006-12-15 发布日期:2011-11-23

Isolation and Toxin Determination of Strains Producing Fumonisin

HOU Hong-Man, ZHANG Xiao, ZHANG Hao, CHEN Li, LIU Yang

College of Biology and Food Technology, Dalian Institute of Light Industry, Dalian 116034, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 伏马菌素(fumonisin)是二十世纪八十年代末期发现的一类由串珠镰刀菌产生的真菌毒素。本文从市售玉米中分离出两株产毒镰刀菌,研究其发酵液中伏马菌素B1的提取、纯化方法,利用薄层层析对伏马菌素B1做半定量检测,利用HPLC法对伏马菌素B1做定量检测。同时,对大连市售玉米花生样品进行了伏马菌素B1的检测。

关键词: 伏马菌素, 毒株分离, 毒素检测

Abstract: The fumonisins are mycotoxin, originally isolated in 1988, synthesized mainly by several species of genus fusuriam. In this paper, two strains of C405 and C510 producing fumonisin were separated from corns, and primarily identified as genus fusarium. The methods of extraction and purification on fumonisin B1 from fermentable liquid were studied. TLC was chosen for half-quantificational determination of fumonisin B1, and HPLC for quantificational determination. Meanwhile, fumonisin B1 was extracted from corns and peanuts was detected too.

Key words: fumonisin, isolation of strains, toxin determination

侯红漫, 张笑, 张昊, 陈莉, 刘阳. 伏马菌素产生菌的分离及其产毒检测[J]. 食品科学, 2006, 27(12): 691-693.

HOU Hong-Man, ZHANG Xiao, ZHANG Hao, CHEN Li, LIU Yang. Isolation and Toxin Determination of Strains Producing Fumonisin[J]. FOOD SCIENCE, 2006, 27(12): 691-693.

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