食品科学 ›› 2013, Vol. 34 ›› Issue (23): 313-316.doi: 10.7506/spkx1002-6630-201323063

• 营养卫生 • 上一篇    下一篇

伏马菌素B1对人正常食管上皮细胞细胞周期相关基因mRNA表达的影响

王少康,王婷婷,黄桂玲,石若夫,杨立刚,董淑楠,孙桂菊*   

  1. 东南大学公共卫生学院营养与食品卫生学系,环境医学工程教育部重点实验室,江苏 南京 210009
  • 收稿日期:2013-10-23 修回日期:2013-11-07 出版日期:2013-12-15 发布日期:2014-01-03
  • 通讯作者: 孙桂菊 E-mail:shaokangwang@gmail.com
  • 基金资助:

    国家自然科学基金项目(30800914;81372985);达能膳食营养研究与宣教基金项目(DIC2011-05);
    江苏省普通高校研究生科研创新计划项目(CXZZ-0179)

Effect of Fumonisin B1 on the mRNA Expressions of Cell Cycle-related Genes in Human Normal Esophageal Epithelial Cells

WANG Shao-kang,WANG Ting-ting,HUANG Gui-ling,SHI Ruo-fu,YANG Li-gang,DONG Shu-nan,SUN Gui-ju*   

  1. Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene,
    School of Public Health, Southeast University, Nanjing 210009, China
  • Received:2013-10-23 Revised:2013-11-07 Online:2013-12-15 Published:2014-01-03
  • Contact: SUN Gui-ju E-mail:shaokangwang@gmail.com

摘要:

本研究采用体外实验,分离鉴定人正常食管上皮细胞,用RT-PCR方法测定伏马菌素B1(fumonisin B1,FB1)对细胞周期相关基因Cyclin D1、Cyclin E、P16、P21、P27 mRNA表达的影响。结果表明:FB1作用人正常食管上皮细胞后可以在转录水平上影响Cyclin D1、Cyclin E、P16、P21、P27基因的表达,导致Cyclin D1 mRNA高表达,Cyclin E、P16、P21、P27基因mRNA低表达。提示,细胞周期相关基因的异常表达与癌症密切相关,该研究从体外实验进一步证实FB1可能与人类食管癌的发生有关。

关键词: 伏马菌素B1, 人正常食管上皮细胞, 细胞周期, 基因表达

Abstract:

Epidemiological studies have shown that fumonisin B1 (FB1), a fungal toxin, may be associated with esophageal
cancer, but the laboratory evidence is still limited. In this study, human normal esophageal epithelial cells were isolated and
identified in vitro. The influence of FB1 on the mRNA expression of cell cycle-related genes including Cyclin D1, Cyclin E, P16,
P21, and P27 was determined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that FB1
played an important role in regulating the expression of Cyclin D1, Cyclin E, P16, P21, and P27 genes in normal human
esophageal epithelial cells at the transcriptional level, leading to high mRNA expression of Cyclin D1 but low mRNA
expression of Cyclin E, P16, P21, and P27 genes. The abnormal expression of cell cycle-related genes is closely related with
cancer. This in vitro study has further confirmed that FB1 is related with the occurrence of human esophageal cancer.

Key words: fumonisin B1, human normal esophageal epithelial cells, cell cycle, gene expression

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