食品科学 ›› 2020, Vol. 41 ›› Issue (12): 113-119.doi: 10.7506/spkx1002-6630-20190505-021

• 生物工程 • 上一篇    下一篇

碳酸钙对凝结芽孢杆菌CGMCC 9951芽孢形成的影响

田晶晶,吴影,李长福,周艳林,周子吕,古绍彬   

  1. (1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.河南省食品微生物工程技术研究中心,河南 洛阳 471023;3.食品加工与安全国家级实验教学示范中心,河南 洛阳 471023)
  • 出版日期:2020-06-25 发布日期:2020-06-22
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401672);河南省自然科学基金项目(182300410066); 河南省科技攻关项目(162102210199)

Effect of CaCO3 on Sporulation of Bacillus coagulans CGMCC 9951

TIAN Jingjing, WU Ying, LI Changfu, ZHOU Yanlin, ZHOU Zilü, GU Shaobin   

  1. (1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China;2. Henan Engineering Research Center of Food Microbiology, Luoyang 471023, China; 3. National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471023, China)
  • Online:2020-06-25 Published:2020-06-22

摘要: 研究碳酸钙添加对凝结芽孢杆菌CGMCC 9951芽孢形成的影响及可能机制。结果表明,在16 h添加6%碳酸钙后,不仅确保了芽孢形成时充足的钙要求,还可促使发酵液pH值由4左右迅速提升至6.5以上,快速启动芽孢的形成。培养(68±2)h,芽孢形成量可达4.53×107 CFU/mL,比对照组芽孢化启动提前约100 h,且芽孢耐热性、耐酸、耐胆盐及其萌发能力均未受显著影响。进一步研究发现,碳酸钙的添加有效加速了凝结芽孢杆菌CGMCC 9951芽孢形成进程,在添加碳酸钙4 h后,芽孢化启动相关基因kinC、kinE、spo0A相对表达量显著提高,分别为对照组的61.07、23.49、17.32 倍。研究表明,凝结芽孢杆菌芽孢形成具有一定的环境pH值依赖性,中性偏碱的环境有利于芽孢化启动相关基因的转录,进而加速芽孢的形成过程。

关键词: 凝结芽孢杆菌, 芽孢形成, 碳酸钙, 芽孢抗性, 基因表达

Abstract: The effect of calcium carbonate on the sporulation of Bacillus coagulans CGMCC 9951 and its possible mechanism were studied in this investigation. The results showed that addition of 6% CaCO3 to the fermentation broth at 16 h not only ensured sufficient supply of calcium for sporulation, but also increased the pH of the fermentation broth from about 4 to over 6.5, quickly initiating sporulation. At (68 ± 2) h, the concentration of spores reached 4.53 × 107 CFU/mL, and sporulation was initiated about 100 hours earlier compared with the control group. Moreover, the heat resistance, acid resistance, bile salt tolerance and germination ability of spores were not significantly affected by CaCO3. Further we found that addition of CaCO3 effectively accelerated the sporulation process. The expression levels of kinC, kinE and spo0A genes related to sporulation initiation were significantly increased at 4 h after adding CaCO3, reaching levels 61.07, 23.49, and 17.32 times as high as the control group, respectively. This study revealed that the sporulation of B. coagulans was dependent upon environmental pH and neutral and weakly alkaline environments were conducive to the transcription of sporulation-related genes, thus accelerating the sporulation process.

Key words: Bacillus coagulans, sporulation, CaCO3, spore resistance, gene expression

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