百里香精油微胶囊对摩根氏菌和变形杆菌产组胺机制的影响

doi:10.7506/spkx1002-6630-20190923-268

食品科学 ›› 2020, Vol. 41 ›› Issue (19): 9-16.doi: 10.7506/spkx1002-6630-20190923-268

百里香精油微胶囊对摩根氏菌和变形杆菌产组胺机制的影响

于红红，吴明颖，王庆玲，董娟，卢士玲

（石河子大学食品学院，新疆?石河子 832000）

出版日期:2020-10-15 发布日期:2020-10-23
基金资助:
国家自然科学基金面上项目（31360392）

Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus

YU Honghong, WU Mingying, WANG Qingling, DONG Juan, LU Shiling

(Food College, Shihezi University, Shihezi 832000, China)

Online:2020-10-15 Published:2020-10-23

摘要/Abstract

摘要： 本实验研究百里香精油微胶囊对产组胺的摩根氏菌ND和变形杆菌R3的生长、基因表达情况以及组胺积累量的影响。利用反转录实时荧光定量聚合酶链式反应分析供试菌在百里香精油微胶囊作用下的组氨酸脱羧途径相关基因的表达情况，采用高效液相色谱仪检测供试菌培养48 h期间的组胺积累量。结果表明，百里香精油微胶囊对两种供试菌有较强的抑菌活性，对摩根氏菌ND和变形杆菌R3的最低抑菌浓度（minimal inhibitory concentration，MIC）分别为1.56 mg/mL和0.78 mg/mL。百里香精油微胶囊可以通过抑制组氨酸脱羧酶（histidine decarboxylase，HDC）簇中HDC基因hdcA和组氨酸/组胺抗逆转录酶基因hdcP的表达从而明显减少组胺的积累。当百里香精油微胶囊的添加量为MIC时，可使摩根氏菌ND组胺积累量降低61.08%，变形杆菌R3组胺积累量降低55.89%。

关键词: 百里香精油微胶囊；摩根氏菌；变形杆菌；组胺；组氨酸脱羧酶；基因表达

Abstract: This study explored the effects of thyme essential oil microcapsules on the growth, gene expression and histamine accumulation of Morganella morganii ND and Proteus bacillus R3. High-performance liquid chromatography was used to detect the accumulation of histamine during 48 h culture of the tested strains, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression of histidine decarboxylase (HDC) pathway-related genes under the influence of thyme essential oil microcapsules. The results showed that thyme essential oil microcapsules had strong antibacterial activity against the test bacteria. The minimal inhibitory concentration (MIC) was 1.56 and 0.78 mg/mL for M. morganii and P. bacillus, respectively. Thyme essential oil microcapsules could reduce histamine accumulation by inhibiting the expression of histidine decarboxylase (hdcA) and histidine/histamine antiporter (hdcP) genes in the HDC cluster. The accumulation of histamine could be reduced by 61.08% and 55.89% in M. morganii and P. bacillus by thyme essential oil microcapsules at MIC concentration.

Key words: thyme essential oil microcapsules; Morganella morganii; Proteus bacillus; histamine; histidine decarboxylase; gene expression

中图分类号:

TS207.7

于红红，吴明颖，王庆玲，董娟，卢士玲. 百里香精油微胶囊对摩根氏菌和变形杆菌产组胺机制的影响[J]. 食品科学, 2020, 41(19): 9-16.

YU Honghong, WU Mingying, WANG Qingling, DONG Juan, LU Shiling. Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus[J]. FOOD SCIENCE, 2020, 41(19): 9-16.

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百里香精油微胶囊对摩根氏菌和变形杆菌产组胺机制的影响

Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus

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