食品科学 ›› 2000, Vol. 21 ›› Issue (7): 40-42.

• 分析检测 • 上一篇    下一篇

化学发光型葡萄糖传感器法测定食品中的葡萄糖

 袁道强, 舒友琴   

  1. 郑州牧业工程高等专科学校
  • 出版日期:2000-07-15 发布日期:2011-12-02

The Determination of Glucose in Food by Chemiluminescence Glucose Sensor

 YUAN  Dao-Qiang, SHU  You-Qin   

  • Online:2000-07-15 Published:2011-12-02

摘要: 报道了一种基于用溶胶凝胶法固定葡萄糖氧化酶(GOD)的化学发光型葡萄糖传感器,此传感器用于流动注射化学发光分析体系,可实现对葡萄糖的在线检测,测定的线性范围为10-1000μmol/L,检出限为0.8μmol/L,10次测定的RSD为1.6%,单次测定能在1min内完成。方法可用于各种食品中葡萄糖含量的测定。

关键词: 溶胶凝胶技术, 固定化, 葡萄糖氧化酶, 化学发光, 葡萄糖

Abstract: In this paper. a kind of chemiluminescence glucose sensor based on GOD glucose oxidase immobilized by solgel was proposed. With the application of the sensor to flowing injection chemiluminescent system, glucose could be effectively determined with the linear range of its concentration in 10~1000μmol/L and detection limit at 0.8 μ mol/L.A complete analysis could be performed in 1 min with RSD (Relative Standard Deviation) of 1 .6% .The system has been applied successfully to the determination of glucose in various food samples.

Key words: Sol-gel technique Immobilization GOD Chemiluminescence Glucose sensor