食品科学 ›› 2012, Vol. 33 ›› Issue (20): 109-112.

• 工艺技术 • 上一篇    下一篇

黄芩茎叶中总黄酮提取工艺优化

史高峰,祝娟娟,张兴潜,陈学福,李沙金   

  1. 兰州理工大学石油化工学院
  • 收稿日期:2011-07-31 修回日期:2012-09-18 出版日期:2012-10-25 发布日期:2012-11-09
  • 通讯作者: 祝娟娟 E-mail:zjj1116jky@163.com

Optimization of Extraction Process for Total Flavonoids from Stems and Leaves of Scutellaria baicalensis Georgi

  • Received:2011-07-31 Revised:2012-09-18 Online:2012-10-25 Published:2012-11-09

摘要: 以黄芩茎叶为原料,采用醇提法提取黄芩茎叶总黄酮。在单因素试验的基础上,以提取温度、提取时间、乙醇体积分数和料液比为考察因素,采用L9(34)正交试验探讨醇提法提取黄芩茎叶总黄酮工艺。最佳工艺条件:以70%乙醇为溶剂、提取温度70℃、料液比1:18(g/mL)、提取3次、每次2.0h,此时黄芩茎叶提取物中总黄酮含量为21.02%,总黄酮得率1.39%。

关键词: 黄芩茎叶, 总黄酮, 提取工艺

Abstract: The extraction of total flavonoids from stems and leaves of Scutellaria baicalensis Georgi by ethanol extraction was optimized using an L9(34) orthogonal array design. One-factor-at-a-time method was used to investigate the effects of temperature, extraction time, ethanol concentration and solid/solvent ratio on extraction efficiency. The optimum conditions for the extraction of total flavonoids were determined as three extraction cycles with 70% ethanol at a solid/solvent ratio of 1:18 (g/mL), 70 ℃ for 2.0 h each. Under these conditions, the yield and purity of crude total flavonoid extract were 21.02% and 1.39%, respectively.

Key words: Scutellaria baicalensis Georgi, total flavonoids, extraction process