食品科学 ›› 2013, Vol. 34 ›› Issue (11): 83-87.doi: 10.7506/spkx1002-6630-201311019

• 基础研究 • 上一篇    下一篇

莲子红衣醇提物的抗氧化和抗褐变活性

高 航,高延芬,徐 虹   

  1. 北京工商大学,食品添加剂与配料北京高校工程研究中心,北京市食品风味化学重点实验室,北京 100048
  • 收稿日期:2012-11-26 修回日期:2013-05-06 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 徐虹 E-mail:xuhong@th.btbu.edu.cn
  • 基金资助:

    北京市优秀人才培养资助项目;十二五科技支撑项目

Antioxidant and Anti-browning Activities of Ethanol Extract from Lotus Seed Skin

GAO Hang,GAO Yan-fen,XU Hong   

  1. Beijing Key Laboratory of Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University, Beijing 100048, China
  • Received:2012-11-26 Revised:2013-05-06 Online:2013-06-15 Published:2013-06-03
  • Contact: XU Hong E-mail:xuhong@th.btbu.edu.cn

摘要:

为深入探索莲子加工副产物红衣粉的应用价值,采用清除ABTS+·能力、抑制蘑菇酪氨酸酶单酚酶和二酚酶活性能力、抗苹果褐变能力指标,综合评价莲子红衣醇提物的抗氧化和抗褐变活性,并测定其总酚和总黄酮含量。结果表明:莲子红衣醇提取物的总酚含量为(233.63±10.16)mg/g,总黄酮含量为(166.02±7.31)mg/g;其清除ABTS+·的IC50为(1.385±0.210)mg/mL;在对蘑菇酪氨酸酶活性的抑制实验中,对单酚酶和二酚酶活性的IC50分别为(1.665±0.137)mg/mL和(3.777±0.294)mg/mL;在鲜切苹果褐变模型中,通过分析L*、a*和b*指数的变化,莲子红衣醇提物表现出了较好的抗褐变性能,尤其当醇提物与0.5g/100mL抗坏血酸组合使用时,其抗褐变效果最佳。

关键词: 莲子红衣醇提物, 抗氧化活性, 抗褐变活性

Abstract:

The goal of the present work was to evaluate the antioxidant and anti-browning activities of ethanol extract
from lotus seed skin. The ABTS+ radical scavenging activity, anti-tyrosinase activity and anti-browning effect as well as the
contents of total phenols and total flavonoids of the extract were determined. The results revealed that the ethanol extract
contained (233.63 ± 10.16) mg/g total phenols and (166.02 ± 7.31) mg/g total flavonoids. It also showed a strong antioxidant
activity against ABTS free radicals with IC50 of (1.385 ± 0.210) mg/mL. In tyrosinase inhibitory assay, the measured IC50
values were (1.665 ± 0.137) mg/mL for monophenolase and (3.777 ± 0.294) mg/mL for diphenolase, respectively. This
extract had a significant anti-browning effect on fresh-cut apple slices as demonstrated by measuring changes in L*, a*and
b* values, and the best efficacy was observed when combined with 0.5 g/100 mL ascorbic acid.

Key words: ethanol extract from lotus seed skin, antioxidation, anti-browning

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