食品科学

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植物激素对纳塔尔链霉菌发酵产纳他霉素的影响

魏宝东,王利艳,于思雯,潘娅慧   

  1. 沈阳农业大学食品学院,辽宁 沈阳 110866
  • 出版日期:2014-06-15 发布日期:2014-07-03

Influences of Plant Hormones on the Natamycin Production of Streptomyces natalensis

WEI Bao-dong, WANG Li-yan, YU Si-wen, PAN Ya-hui   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Online:2014-06-15 Published:2014-07-03

摘要:

以纳塔尔链霉菌(Streptomyces natalensis)为发酵菌种,在摇瓶培养基中添加植物激素,通过单因素试验初筛,获得能够有效提高纳他霉素的产量的植物激素;采用Box-Behnken试验设计建立数学模型,进行响应面分析优化确定最优组合。结果表明:吲哚乙酸、赤霉素和6-苄氨基嘌呤均能够有效提高纳他霉素的产量,在5.5 mg/L吲哚乙酸、14.8 mg/L赤霉素和20.5 mg/L 6-苄氨基嘌呤的条件下发酵120 h时,发酵液中纳他霉素的发酵产量可达到1.805 g/L,为对照组的1.63 倍。

关键词: 植物激素, 纳塔尔链霉菌, 纳他霉素, 发酵, 响应面分析

Abstract:

Screening of five plant hormones was carried out to find out those that can effectively enhance natamycin
production in shake-flask culture by Streptomyces natalensis upon addition to the medium. A mathematical model was built
using Box-Behnken experimental model and used to determine the optimal concentrations of selected plant hormones in the
medium. The results showed that indole-3-acetic acid, gibberellin and 6-benzyl amino purine effectively increased natamycin
production. At optimized concentrations of 5.5, 14.8 and 20.5 mg/L for these plant hormones, respectively, the yield of
natamycin was 1.805 g/L, which was 1.63 times higher than that of the control group.

Key words: plant hormones, Streptomyces natalensis natamycin, fermentation, response surface methodology