外源镧对不同黄瓜品种镉积累及镉化学形态的影响

摘要/Abstract

摘要： 重金属镉（Cd）是土壤的重要污染物之一，其对土壤生态系统造成的危害已引起学者的广泛关注。本研究采用盆栽试验模拟了土壤重金属镉（20 mg · kg-1)污染下，外源镧（0、10和20 mg·L-1 LaCl3）对‘燕白’和‘津优1号’2个品种黄瓜(Cucumis satiuus L. )生长、丙二醛（MDA）含量、抗氧化酶活性及果实中的总镉含量和形态的影响。结果表明，镧提高了2个品种叶和根的抗氧化酶（CAT、SOD和POD）的活性，增加了2个品种的叶、茎、根、果实干质量及植株总干质量。镧对‘津优1号’植株干物质量的影响大于‘燕白’。外源镧使黄瓜叶和根抗氧化酶活性升高可能是植株对镉的抗性增强、干质量增加的生理机制之一。镧减少了2个品种果实中总Cd含量和不同形态Cd含量，但随着镧浓度的增加，2个品种果实总Cd含量和残渣态Cd（FR）含量表现为先降后升的趋势。外源镧使黄瓜叶、茎、根和果实中的Cd含量分别降低了6.0%~10.2%、8.9%~23 .5%、4.0%~29.2%和32.0%~49.8%。喷镧后，单株果实的Cd含量和Cd积累量、植株Cd全量均以‘津优1号’>‘燕白’。

关键词: 镧镉交互, 抗氧化酶, 丙二醛, 镉累积, 镉形态, 黄瓜品种

Abstract: Cadmium (Cd) is one of the most important pollutants in soil, and it has attracted wide attention of scientists because of its harm to soil ecosystem. Pot experiments were carried out to investigate the effects of different lanthanum (La) levels（0,10 and 20 mg·L-1 LaCl3)on dry weights of plant, concentration of malonaldehyde ( MDA), activities of antioxidant enzymes, accumulation and chemical forms of cadmium（Cd）in two cucumber varieties ('Yanbai' and 'Jinyou 1' ) when exposed to Cd（20 mg·kg-1）. The results showed that activities of CAT, SOD and POD in leaf and root , and dry weights of leaf, stew, root, fruit and plant of two varieties increased in the presence of exogenous LaCl3 when exposed to Cd. Effect of La on dry weights of plant was found higher in 'Jinyou 1' than that in'Yanbai'. Exogenous lanthanum, which can increase antioxidant enzyme activities of leaf and root in two cucumber varieties, may play an important role in increasing resistance of plant to Cd and dry weights of plant.All chemical forms of Cd and the total extractable Cd in fruit of two varieties obviously decreased in LaCl3 treatments compared to the control, while the total extractable Cd and residual Cd (FR) of two varieties deceased at first, and then increased with the increase of LaCl3 level. Cadmium concentration in leaf, stem, roots and fruit of both varieties decreased by 8.0%-10.2%, 8.9%-23 .5%, 4.0%-29.2% and 32.0%-49.8% in the presence of LaCl3 when exposed to Cd. Cadmium concentration of fruit, and Cd accumulations in fruit and plant were in order of 'Jinyou 1' >'Yanbai' in the presence of LaCl3.

Key words: Antagonism of lanthanum and cadmium, concentration of malonaldehyde, activities of antioxidant enzymes, Cd accumulation, Cd fractions, cucumber varieties

中图分类号:

X171.5

陈蓉徐卫红. 外源镧对不同黄瓜品种镉积累及镉化学形态的影响[J]. 食品科学, 0, (): 0-0.

Chen Rong. Effect of Exogenous Lanthanum on on Accumulation and Chemical Forms of Cadmium in Different Varieties of Cucumber(Cucumis satiuus L. )[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]袁祖丽, 马新明, 韩锦峰, 等.镉污染对烟草叶片超微结构及部分元素含量的影响[J].生态学报, 2005, 25(11):2919-2927 [2]刘俊, 周坤, 徐卫红, 等.不同浓度外源铁对番茄生理特性、镉积累及化学形态的影响[J].环境科学, 2013, 34(10):4126-4131 [3]Wang C L, Xu W H, LI H, et al.Effects of zinc on physiologic characterization and cadmium accumulation and chemical forms in different varieties of pepper[J].Wuhan University Journal of Natural Sciences, 2013, 18(6):541-548 [4]Satarug S, Baker J R, Urbenjapol S, et al.A global perspective on cadmium pollution and toxicity in non-occupationally exposed population[J].Toxicology Letters, 2003, 137(1-2):65-83 [5]曾希柏, 李莲芳, 梅旭荣.中国蔬菜土壤重金属含量及来源分析[J].中国农业科学, 2007, 40(11):2507-2517 [6]刘意章, 肖唐付, 宁增平, 等.三峡库区巫山建坪地区土壤镉等重金属分布特征及来源研究[J].环境科学, 2013, 34(6):2390-2398 [7] 方华为.不同品种蔬菜对镉的吸收及根系形态特征研究[D]. 武汉: 华中农业大学, 2011. [8]陈贵青, 张晓璟, 徐卫红, 等.不同水平下辣椒体内的积累、化学形态及生理特性[J].环境科学, 2010, 31(7):247-252 [9]陈惠, 曹秋华, 徐卫红, 等.镉对不同品种辣椒幼苗生理特性及镉积累的影响[J].西南师范大学学报自然科学版, 2013, 38(9):110-115 [10]宋波, 陈同斌, 郑袁明, 等.北京市菜地土壤和蔬菜镉含量及其健康风险分析[J].环境科学学报, 2006, 26(8):1343-1353 [11]陈红亮, 龙　黔, 谭　红.贵州北部菜地土壤镉含量与蔬菜镉污染的关系[J].四川农业大学学报, 2011, 29(3):342-352 [12]McGrath SP, Lombi E, Gray CW, et al.Field evaluation of Cd and Zn phytoextraction potential by the hyperaccumulators Thlaspi caerulescens and Arabidopsis halleri[J].Environmental Pollution, 2006, 141(1):115-125 [13]Sorial M E, Abd El-Fattah M A.Alleviation of the adverse effects of lead and cadmium on growth and chemical changes of pea plant by phosphorus and calcium treatments[J].Journal of Pest Control and Environmental Sciences, 2001, 9(1):161-194 [14]周青, 张辉, 黄晓华, 等.镧对镉胁迫下菜豆幼苗生长的影响[J].环境科学, 2003, 24(4):48-53 [15] 张杰, 黄永杰, 刘雪云.镧对镉胁迫下水稻幼苗生长及生理特性的影响[J]. 生态环境 2007, 16(3): 835-841. [16]庞欣, 王东红, 彭安.镧对铅胁迫下小麦幼苗抗氧化酶活性的影响[J].环境化学, 2002, 21(4):318-323 [17] Xiong S L, Xiong Z T, Chen Y C, et a1.Interactive effects of lanthanum and cadmium on plant growth and mineral element uptake in crisped-leaf mustard under hydroponic conditions[J].Journal of Plant Nutrition, 2006, 29:1889-1902. [18] 张海波.不同辣椒品种镉积累差异及外源物质对镉富集的调控效应[D]. 重庆: 西南大学, 2013. [19]李德明, 朱祝军, 钱琼秋.白菜镉积累基因型差异研究[J].园艺学报, 2004, 31(1):97-98 [20]孙建云, 沈振国.镉胁迫对不同甘蓝基因型光合特性和养分吸收的影响[J].应用生态学报, 2007, 18(11):2605-2610 [21]朱芳, 方炜, 杨中艺.番茄吸收和积累能力的品种间差异[J].生态学报, 2005, 26(12):4071-4081 [22] 张媛媛.重金属镉对黄瓜幼苗生理特性的影响及嫁接缓解镉毒害的生理机制研究[D]. 武汉: 华中农业大学, 2009. [23]张琼.镉对黄瓜幼苗生长的影响[J].漳州师范学院学报自然科学版, 2009, 64(2):118-122 [24] 鲁如坤.土壤农业化学分析方法[M] . 北京: 中国农业科技出版社, 2000. 12-22, 107-195, 335-336. [25] 李合生.植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000, 165-167. [26] 张志良.植物生理学实验指导[M], 北京: 高等教育出版社, 2002, 154-155. [27]Alarcón A L, Madrid R, Romojaro F, et al.Calcium forms in leaves of muskmelon plants grown with different calcium compounds[J].Journal of Plant Nutrition, 1998, 21(9):1897-1912 [28]张晓璟, 刘吉振, 徐卫红, 等.磷对不同辣椒品种镉积累、化学形态及生理特性的影响[J].环境科学, 2011, 32(4):1171-1176 [29]晏文武, 杨利民, 王秋泉.镧在菠菜叶绿体中的分布及其对光合作用的影响[J].科学通报, 2005, 50(12):1195-1200 [30] 史沛丽, 张玉秀, 柴团耀.龙葵叶片和根系抗氧化酶对镉胁迫的响应[J].. 中国科学院大学学报, 2013, 30(5); 608-612, 643. [31] 王友保, 张　莉, 沈章军, 等.2005. 铜尾矿库区土壤与植物中重金属形态分析[J].. 应用生态学报, 16 (12): 2418-2422. [32]张智勇, 王玉琦, 孙景信, 等.分子活化分析研究植物原生质体中的稀土元素[J].科学通报, 2000, 45(5):502-505 [33]彭伟正, 王克勤, 胡蝶, 等.镉在黄瓜植株体内分布规律及其对黄瓜生长和某些生理特性的影响[J].农业环境科学学报, 2006, 25(增刊):92-95 [34] 沙莎.Hg、Cd胁迫下稀土元素La对豌豆及玉米幼苗的影响[D]. 南京: 南京师范大学, 2004.