中强度脉冲电场对娃娃菜活性物质含量及抗氧化能力的影响

doi:10.7506/spkx1002-6630-20200914-178

食品科学 ›› 2021, Vol. 42 ›› Issue (19): 183-193.doi: 10.7506/spkx1002-6630-20200914-178

• 食品工程 • 上一篇

中强度脉冲电场对娃娃菜活性物质含量及抗氧化能力的影响

张映曈，张元元，赵欢欢，胡花丽，张雷刚，周宏胜，罗淑芬，李鹏霞

（1.江苏省农业科学院农业设施与装备研究所，江苏南京 210014；2.南京农业大学食品科学技术学院，江苏南京 210095；3.江苏省高效园艺作物遗传改良重点实验室，江苏南京 210014）

发布日期:2021-11-12
基金资助:
江苏省重点研发计划面上项目（BE2018383）；江苏省农业科技自主创新资金项目（CX（18）2028）

Effect of Moderate Intensity Pulsed Electric Field on the Contents of Bioactive Compounds and Antioxidant Capacity of Baby Cabbage (Brassica pekinensis L. var. gemmifera Zenk.)

ZHANG Yingtong, ZHANG Yuanyuan, ZHAO Huanhuan, HU Huali, ZHANG Leigang, ZHOU Hongsheng, LUO Shufen, LI Pengxia

(1. Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 3. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)

Published:2021-11-12

摘要/Abstract

摘要： 为探明中强度脉冲电场（moderate intensity pulsed electric fields，MIPEF）诱导果蔬代谢的应激机制，同时确定最佳处理条件以获得高活性物质含量的娃娃菜，本实验研究不同电场强度（0.4～2.0 kV/cm）和占空比（0%～49.2%）的MIPEF处理对娃娃菜活性物质含量、抗氧化酶系（过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶）活性以及抗氧化能力的影响。结果表明，MIPEF可刺激娃娃菜酚类物质、抗坏血酸、谷胱甘肽、总硫代葡萄糖苷和异硫氰酸酯的合成（2.76%～818.03%），同时显著提升抗氧化酶系的活性（2.22%～42.00%），从而提高1,1-二苯基-2-三硝基苯肼（1,1-dipenyl-2-pierylhydrazyl，DPPH）自由基、羟自由基（·OH）和超氧阴离子自由基（O2－·）清除率和总还原力（1.81%～202.11%），以延缓娃娃菜衰老进程。通过拟合预测和验证实验，得出最优处理条件为电场强度1.1 kV/cm、占空比46.4%，此时活性物质含量为理论预测值的88.91%～103.40%。结论：MIPEF处理可显著提升娃娃菜活性物质含量和抗氧化能力，是一种极具潜力的果蔬非热预处理方式。

关键词: 中强度脉冲电场；娃娃菜；活性物质；抗氧化酶；抗氧化能力

Abstract: In order to clarify the metabolic response mechanism of fruits and vegetables to moderate intensity pulsed electric field (MIPEF) and to determine the optimal treatment conditions for increased contents of bioactive compounds in baby cabbage, the effects of different electric field intensities (0.4–2.0 kV/cm) and duty ratios (0%–49.2%) on the contents of bioactive compounds, the activity of antioxidant enzymes and antioxidant capacity in baby cabbage were investigated. The results showed that MIPEF increased the contents of bioactive compounds including phenols, ascorbic acid, glutathione, total glucosinolates and isothiocyanates by 2.76%–818.03% compared with the control group, and enhanced the activity of antioxidant enzymes including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) by 2.22%–42.00%, and the scavenging activity toward 1,1-dipenyl-2-pierylhydrazyl (DPPH), hydroxyl and superoxide anion radical as well as total reducing power by 1.81%–202.11%, thus delaying the senescence process. The results of model prediction and validation experiments showed that the optimized treatment conditions were found to be 1.1 kV/cm and 46.4% for electric field intensity and duty ratio, respectively, under which the predicted levels of bioactive compounds in MIPEF treated baby cabbage were 88.91%–103.40%. In conclusion, MIPEF treatment can significantly increase the contents of bioactive compounds and antioxidant capacity in baby cabbage, thus being a promising candidate for non-thermal processing of fruits and vegetables.

Key words: moderate intensity pulsed electric field (MIPEF); baby cabbage; bioactive compounds; antioxidant enzymes; antioxidant capacity

中图分类号:

TS255.1

张映曈，张元元，赵欢欢，胡花丽，张雷刚，周宏胜，罗淑芬，李鹏霞. 中强度脉冲电场对娃娃菜活性物质含量及抗氧化能力的影响[J]. 食品科学, 2021, 42(19): 183-193.

ZHANG Yingtong, ZHANG Yuanyuan, ZHAO Huanhuan, HU Huali, ZHANG Leigang, ZHOU Hongsheng, LUO Shufen, LI Pengxia. Effect of Moderate Intensity Pulsed Electric Field on the Contents of Bioactive Compounds and Antioxidant Capacity of Baby Cabbage (Brassica pekinensis L. var. gemmifera Zenk.)[J]. FOOD SCIENCE, 2021, 42(19): 183-193.

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中强度脉冲电场对娃娃菜活性物质含量及抗氧化能力的影响

Effect of Moderate Intensity Pulsed Electric Field on the Contents of Bioactive Compounds and Antioxidant Capacity of Baby Cabbage (Brassica pekinensis L. var. gemmifera Zenk.)

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