外源γ-氨基丁酸对发芽大豆酚类物质富集及抗氧化能力的影响

doi:10.7506/spkx1002-6630-20200614-189

食品科学 ›› 2021, Vol. 42 ›› Issue (13): 72-78.doi: 10.7506/spkx1002-6630-20200614-189

外源γ-氨基丁酸对发芽大豆酚类物质富集及抗氧化能力的影响

丁羽萱，王尧，姚羿安，李皖梅，王冕，王沛，顾振新，杨润强

（南京农业大学食品科学技术学院，江苏南京 210095）

出版日期:2021-07-15 发布日期:2021-07-27
基金资助:
国家级大学生创新项目（202010307042Z）；南京农业大学大学生创新创业训练专项（S20190022）

Effect of Exogenous γ-Aminobutyric Acid on the Accumulation of Phenolics and Antioxidant Capacity in Germinated Soybean

DING Yuxuan, WANG Yao, YAO Yi’an, LI Wanmei, WANG Mian, WANG Pei, GU Zhenxin, YANG Runqiang

(College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)

Online:2021-07-15 Published:2021-07-27

摘要/Abstract

摘要： 种子萌发过程中γ-氨基丁酸（γ-aminobutyric acid，GABA）和酚类物质均得到富集，GABA可能在酚类物质富集中起调控作用。因此，本实验采用水培法，通过对大豆外源添加0、2.5、5、10 mmol/L和20 mmol/L的GABA，测定发芽大豆的生长指标、总酚含量、酚酸含量及抗氧化能力，以研究GABA对发芽大豆酚类物质富集及抗氧化能力的影响。结果表明：2.5、5 mmol/L外源GABA能显著提高发芽大豆的芽长和鲜质量（P＜0.05），促进发芽大豆的生长。随着GABA浓度升高，发芽大豆的总酚和游离酚酸含量逐渐增加。当GABA浓度为20 mmol/L时，总酚含量为对照的1.39 倍。而结合酚酸含量呈现增加后降低的趋势，当GABA浓度为2.5 mmol/L时最高。说明随着GABA浓度的升高，结合酚酸逐渐释放，并向游离形态转化。此外，GABA处理还能够提高发芽大豆的1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)阳离子自由基清除能力和苯丙氨酸解氨酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶活力。综上，外源GABA能显著促进发芽大豆生长、调节苯丙氨酸解氨酶活性和诱导酚类物质累积，同时增强抗氧化酶活力和体外自由基清除能力。研究结果可为开发富含酚类物质的发芽大豆提供信号分子理论参考。

关键词: γ-氨基丁酸；总酚；酚酸；抗氧化能力

Abstract: During seed germination, γ-aminobutyric acid (GABA) and phenolics are both accumulated, and GABA may play a regulatory role in the accumulation of phenolics. In this study, the effect of exogenous GABA at doses of 0, 2.5, 5, 10 and 20 mmol/L was investigated on the growth indices, total phenolics and phenolic acid contents and antioxidant capacity of germinated soybean grown in hydroponics. The results showed that 2.5, 5 mmol/L exogenous GABA could significantly increase the sprout length, mass, growth (P < 0.05). With the increase in GABA concentration, the contents of total phenolics and free phenolic acid increased gradually. When the concentration of GABA was 20 mmol/L, the content of total phenolics was enhanced by 1.39 times relative to the control group. However, the content of bounded phenolic acid increased firstly and then decreased with the increase in GABA concentration, reaching the highest value at 2.5 mmol/L concentration, proving that bound phenolic acid was released gradually and transformed into a free form. In addition, GABA treatment also improved the scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl, 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) cation radical and the activities of peroxidase, catalase and ascorbic acid peroxidase. The above findings indicated that exogenous GABA significantly promoted the growth of germinated soybean, regulated the activity of phenylalanine ammonia lyase, induced the accumulation of phenolics, and enhanced the activity of antioxidant enzymes as well as the free radical scavenging capacity in vitro, which will provide a theoretical basis for the development of soybean sprouts rich in phenolics.

Key words: γ-aminobutyric acid; total?phenolics; phenolic?acid; antioxidant capacity

中图分类号:

TS255.1

丁羽萱，王尧，姚羿安，李皖梅，王冕，王沛，顾振新，杨润强. 外源γ-氨基丁酸对发芽大豆酚类物质富集及抗氧化能力的影响[J]. 食品科学, 2021, 42(13): 72-78.

DING Yuxuan, WANG Yao, YAO Yi’an, LI Wanmei, WANG Mian, WANG Pei, GU Zhenxin, YANG Runqiang. Effect of Exogenous γ-Aminobutyric Acid on the Accumulation of Phenolics and Antioxidant Capacity in Germinated Soybean[J]. FOOD SCIENCE, 2021, 42(13): 72-78.

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外源γ-氨基丁酸对发芽大豆酚类物质富集及抗氧化能力的影响

Effect of Exogenous γ-Aminobutyric Acid on the Accumulation of Phenolics and Antioxidant Capacity in Germinated Soybean

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