食品科学

• 生物工程 • 上一篇    下一篇

玉米秸秆糖醇发酵产丁二酸及表征

叶小金,王红蕾,王晓俊,徐洪章,薛冬桦*   

  1. 长春工业大学化学与生命科学学院,吉林 长春 130012
  • 发布日期:2014-12-15

Fermentation and Characterization of Succinic Acid from Sugar-Alcohol Solution of Corn Stover

YE Xiao-jin, WANG Hong-lei, WANG Xiao-jun, XU Hong-zhang, XUE Dong-hua*   

  1. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China
  • Published:2014-12-15

摘要:

以玉米秸秆糖醇液为原料,考察产琥珀酸放线杆菌(Actinobacillus succinogenes)X-1对不同单一碳源的同化能力,并验证产琥珀酸放线杆菌可同化利用玉米秸秆糖醇液。利用Box-Behnken中心组合设计试验,通过响应面分析法优化发酵工艺参数为:玉米秸秆糖醇液初始还原糖质量浓度42.81 g/L、酵母膏质量浓度12.53 g/L、缓冲剂MgCO3质量浓度15.90 g/L,经5 L发酵规模实验,发酵周期48 h,丁二酸产率为85.1%,还原糖利用率为85.4%。经红外光谱和核磁共振表征其发酵产物为生物基丁二酸。

关键词: 丁二酸, 发酵, 玉米秸秆糖醇液, 产琥珀酸放线杆菌

Abstract:

In this work, the sugar-alcohol solution of corn stover was fermented to produce succinic acid. The carbon
assimilation ability of Actinobacillus succinogenes X-1 was investigated using different carbon sources and the strain was
verified to be able to assimilate the sugar-alcohol solution to produce succinic acid. A Box-Behnken design was used to
optimize three medium components by response surface methodology. The optimum medium was obtained by adding
12.53 g/L yeast extract as a nitrogen source, and 15.90 g/L MgCO3 as a pH buffer to the sugar-alcohol solution containing
42.81 g/L reducing sugar. After 48 h of fermentation in a 5-L fermentor, the yield of succinic acid was 85.1% and the
utilization rate of reducing sugar was 85.4%. The crystal of the fermentation product was obtained by acidic crystallization
and succinic acid was characterized using infrared spectroscopy and nuclear magnetic resonance.

Key words: succinic acid, fermentation, sugar-alcohol solution of corn stover, Actinobacillus succinogenes

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