黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平

doi:10.7506/spkx1002-6630-201509025

食品科学

黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平

马娜1,2，申婷婷2，赵江1，王佳楠3，张泽生1，王浩1,*

1.天津科技大学食品工程与生物技术学院，天津 300457；2.天津科技大学生物工程学院，天津 300457；
3.南开大学滨海学院，天津 300270

出版日期:2015-05-15 发布日期:2015-05-11

Black Rice Reduces Cholesterol Level by Upregulating ABCG5/8 and ABCA1 mRNA Expression in Intestines

MA Na1,2, SHEN Tingting2, ZHAO Jiang1, WANG Jianan3, ZHANG Zesheng1, WANG Hao1,*

1. School of Food Engineering and Biological Technology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
3. Binhai College, Nankai University, Tianjin 300270, China

Online:2015-05-15 Published:2015-05-11

摘要/Abstract

摘要：

以高脂膳食饲喂致高胆固醇小鼠为动物模型，研究黑米对小鼠血脂水平及小肠胆固醇代谢相关基因调控的影响。将48 只雄性C57BL/6J小鼠随机分成高脂对照组和3 个实验组（白米组、黑米低剂量组、黑米高剂量组），测定血清中总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）及高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）水平，气相色谱法检测肝脏中胆固醇含量，实时荧光定量聚合酶链式反应（real-time polymerase chain reaction，real-time PCR）分析调控小肠胆固醇合成、吸收、转化及排泄基因HMG-CoA-R、MTP、ABCG5/8、ABCA1、NPC1L1等的mRNA表达水平。结果表明：与高脂对照组相比，白米组小鼠血清中TC、TG、HDL-C含量无统计学差异，但黑米低、高剂量组小鼠血清中TC和TG含量降低，且HDL-C含量显著或极显著升高（P＜0.05或P＜0.01）。与高脂对照组相比，白米组小鼠肝脏中胆固醇含量无显著变化，黑米低剂量组显著降低（P＜0.05），黑米高剂量组极显著降低（P＜0.01）。Real-time PCR结果显示，与高脂对照组相比，黑米低、高剂量组ABCG5/8、ABCA1 mRNA表达水平均极显著上调（P＜0.01）；黑米低剂量组NPC1L1 mRNA表达水平降低（P＜0.05），黑米高剂量组极显著降低（P＜0.01）。黑米对高脂膳食饲喂致高胆固醇小鼠胆固醇代谢平衡的调节可能是通过增加小肠中胆固醇的排泄并抑制其吸收实现的。

关键词: 黑米, 小鼠, ABCG5/8, ABCA1, NPC1L1

Abstract:

The influence of black rice on serum lipid (TC, TG, HDL-C) levels and the expression of intestinal cholesterol
metabolism-related genes (HMG-CoA-R, MTP, ABCG5/8, ABCA1 and NPC1L1) induced by high fat and high cholesterol
diet (HFHC) was investigated in mice. Male mice (n = 48) were divided into four groups. They were fed a HFHC
diet alone (C group, n = 12), or supplemented with 52.6 g/100 g corn starch or with white rice (C+W group, n = 12),
26.3 g/100 g black rice (C+BL group, n = 12) or 52.6 g/100 g black rice (C+BH group, n = 12). After administration for 8
weeks, blood samples were collected for lipid measurements and viscera were removed at the end of the protocol. Serum
total cholesterol (TC), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) were assayed by commercial
kits. Gas chromatography (GC) was used to determine cholesterol content in hepatic cells. The mRNA expression levels of
intestinal HMG-CoA-R, MTP, ABCG5/8, ABCA1 and NPC1L1 were detected by real-time PCR. The contents of serum TC,
TG and HDL-C in the experimental groups were similar with those of the control group. However, HDL-C in the black rice
groups was significantly elevated when compared with that of the control group (P < 0.05 or P < 0.01). Hepatic cholesterol
content were significantly decreased in the C + BL（P < 0.05）and C + BH（P < 0.01）groups when compared with that
of the C group. The mRNA expression levels of ABCG5/8 and ABCA1 in intestines were up-regulated by dietary back rice
(P < 0.01) when compared with those of the C group. The expression level of NPC1L1 mRNA was downregulated in the
C + BL (P < 0.05) and C + BH (P < 0.01) groups. The increase of cholesterol excretion and the inhibition of cholesterol
absorption in intestines may explain the mechanisms underlying the hypolipidemic effect of black rice.

Key words: black rice, mice, ABCG5/8, ABCA1, NPC1L1

中图分类号:

TS201.4

马娜1,2，申婷婷2，赵江1，王佳楠3，张泽生1，王浩1,*. 黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平[J]. 食品科学, doi: 10.7506/spkx1002-6630-201509025.

MA Na1,2, SHEN Tingting2, ZHAO Jiang1, WANG Jianan3, ZHANG Zesheng1, WANG Hao1,*. Black Rice Reduces Cholesterol Level by Upregulating ABCG5/8 and ABCA1 mRNA Expression in Intestines[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201509025.

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黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平

Black Rice Reduces Cholesterol Level by Upregulating ABCG5/8 and ABCA1 mRNA Expression in Intestines

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