食品科学 ›› 2019, Vol. 40 ›› Issue (21): 163-170.doi: 10.7506/spkx1002-6630-20180927-291

• 营养卫生 • 上一篇    下一篇

黑蒜粉膳食补充对环磷酰胺诱导BALB/c小鼠免疫功能的影响

杨明,秦烨,郝俊宇,吴涛,刘锐,隋文杰,张民   

  1. (天津科技大学食品工程与生物技术学院,食品生物技术教育部工程研究中心,新农村发展研究院,天津 300457)
  • 出版日期:2019-11-15 发布日期:2019-12-02
  • 基金资助:
    天津市教委科研计划重点项目(2017ZD02);天津市科技计划项目(18PTZWHZ00080)

Dietary Supplementation with Black Garlic Powder Exerts Immunostimulatory Activity in Cyclophosphamide Induced BALB/c Mice

YANG Ming, QIN Ye, HAO Junyu, WU Tao, LIU Rui, SUI Wenjie, ZHANG Min   

  1. (Engineering Research Center of Food Biotechnology, Ministry of Education, Institute for New Rural Development, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China)
  • Online:2019-11-15 Published:2019-12-02

摘要: 目的:研究黑蒜粉对BALB/c小鼠的免疫调节作用。方法:采用环磷酰胺建立免疫抑制BALB/c小鼠动物模型,检测不同剂量(100、300、900 mg/kg)黑蒜粉对免疫抑制BALB/c小鼠脾脏、胸腺指数、脾淋巴细胞转化活性、迟发型变态反应能力、抗体生成细胞水平、半数溶血值、碳粒廓清速率、脾细胞中白细胞介素(interleukin,IL)-8和IL-12 mRNA表达水平等指标的影响。结果:与模型组相比,黑蒜粉低、中、高剂量组显著提高了小鼠的胸腺指数、抗体生成能力、脾淋巴细胞增殖能力以及脾细胞因子IL-8和IL-12 mRNA的表达量(P<0.05);中、高剂量组显著提升了小鼠的脾脏指数和迟发型变态反应能力(P<0.05);高剂量组则显著增强了小鼠的血清溶血素水平和单核-巨噬细胞吞噬活性(P<0.05)。结论:黑蒜粉具有提高BALB/c小鼠免疫功能的作用。

关键词: 黑蒜粉, BALB/c小鼠模型, 免疫调节

Abstract: The present study aimed to investigate the immunostimulatory activity of black garlic powder on immunosuppressed BALB/c mice. Cyclophosphamide was injected to establish an immunosuppressed model. Then, the effect of black garlic powder at doses of 100, 300 and 900 mg/kg on splenic and thymus index, the transformation of splenic lymphocytes, delayed type hypersensitivity, quantity of antibody-producing cells, half hemolysis value (HC50), carbon clearance rate, interleukin (IL)-8 and IL-12 gene expression levels in splenic cells of mice were investigated. The results indicated that all three doses of black garlic powder significantly improved thymus index, antibody production, splenic lymphocyte proliferation and the expression of IL-8 and IL-12 in splenic cells when compared with the model control (MC) group (P < 0.05). In addition, compared with the MC group, black garlic powder at the middle and high doses could significantly enhance splenic index and delayed-type hypersensitivity response (P < 0.05). Moreover, compared with the MC group, black garlic powder at the high dose effectively improved serum hemolysin level and mononuclear macrophage phagocytosis activity (P < 0.05). Thus, black garlic powder appears to be an excellent candidate to improve immune function in BALB/c mice.

Key words: black garlic powder, BALB/c mouse model, immunostimulatory

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