关键词: 黑蒜粉, BALB/c小鼠模型, 免疫调节

Abstract: The present study aimed to investigate the immunostimulatory activity of black garlic powder on immunosuppressed BALB/c mice. Cyclophosphamide was injected to establish an immunosuppressed model. Then, the effect of black garlic powder at doses of 100, 300 and 900 mg/kg on splenic and thymus index, the transformation of splenic lymphocytes, delayed type hypersensitivity, quantity of antibody-producing cells, half hemolysis value (HC50), carbon clearance rate, interleukin (IL)-8 and IL-12 gene expression levels in splenic cells of mice were investigated. The results indicated that all three doses of black garlic powder significantly improved thymus index, antibody production, splenic lymphocyte proliferation and the expression of IL-8 and IL-12 in splenic cells when compared with the model control (MC) group (P < 0.05). In addition, compared with the MC group, black garlic powder at the middle and high doses could significantly enhance splenic index and delayed-type hypersensitivity response (P < 0.05). Moreover, compared with the MC group, black garlic powder at the high dose effectively improved serum hemolysin level and mononuclear macrophage phagocytosis activity (P < 0.05). Thus, black garlic powder appears to be an excellent candidate to improve immune function in BALB/c mice.

Key words: black garlic powder, BALB/c mouse model, immunostimulatory