辣白菜中乳酸菌的分离鉴定

doi:10.7506/spkx1002-6630-201511025

食品科学

辣白菜中乳酸菌的分离鉴定

孟令帅1，张颖1，邹婷婷1，乌日娜1,2，岳喜庆1，武俊瑞1,*

1.沈阳农业大学食品学院，辽宁沈阳 110866；2.江南大学食品学院，食品科学与技术国家重点实验室，江苏无锡 214122

出版日期:2015-06-15 发布日期:2015-06-07

Isolation and Identification of Lactic Acid Bacteria from Kimchi

MENG Lingshuai1, ZHANG Ying1, ZOU Tingting1, WU Rina1,2, YUE Xiqing1, WU Junrui1,*

1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Online:2015-06-15 Published:2015-06-07

摘要/Abstract

摘要：

从沈阳市7 个区25 份朝鲜族家庭制作的传统发酵辣白菜中分离出81 株乳酸菌疑似菌株，初步鉴定34 株为杆菌，47 株为球菌。进一步采用16S rDNA序列分析对81 株菌进行分子鉴定，通过序列分析进行属种鉴定。结果表明：81 株菌均为乳酸菌，分别来自2 个属6 个种，45 株为屎肠球菌，25 株为植物乳杆菌，4 株为干酪乳杆菌，3 株为戊糖乳杆菌，2 株为短乳杆菌，2 株为坚强肠球菌。研究结果为我国东北辣白菜中乳酸菌作进一步研究奠定了基础。

关键词: 辣白菜, 乳酸菌, 16S rDNA, 同源性分析

Abstract:

Lactic acid bacteria were isolated and identified from 25 Korean traditional fermented kimchi samples from
7 districts of Shenyang. Totally 81 strains suspected of being lactic acid bacteria were isolated successfully, and 34 of
the isolated strains were identified as bacilli and 47 were identified as cocci. These 81 strains were further identified by
16S rDNA sequence analysis. The results showed that all these 81 strains were confirmed as lactic acid bacteria from 2 genus
and 6 species, including 45 strains of Enterococcus faecium, 25 strains of Lactobacillus plantarum, 4 strains of Lactobacillus
casei, 3 strains of Lactobacillus pentosus, 2 strains of Lactobacillus brevis, and 2 strains of Enterococcus durans. These
results can provide a basis for further studies on lactic acid bacteria from kimchi in northeast China.

Key words: kimchi, Lactobacillus, 16S rDNA, homology analysis

中图分类号:

TS201.3

孟令帅1，张颖1，邹婷婷1，乌日娜1,2，岳喜庆1，武俊瑞1,*. 辣白菜中乳酸菌的分离鉴定[J]. 食品科学, doi: 10.7506/spkx1002-6630-201511025.

MENG Lingshuai1, ZHANG Ying1, ZOU Tingting1, WU Rina1,2, YUE Xiqing1, WU Junrui1,*. Isolation and Identification of Lactic Acid Bacteria from Kimchi[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201511025.

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辣白菜中乳酸菌的分离鉴定

Isolation and Identification of Lactic Acid Bacteria from Kimchi

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