关键词: 分子印迹聚合物, 表面分子印迹技术, 正交试验设计, 乙氧酰胺苯甲酯

Abstract: The novel functional monomer which named N,O-bismethacryloyl ethanolamine was synthesized. ETP-MIPs were prepared by surface molecular imprinting technique with the silica gel as a support matrix and ethylene glycol dimethacrylate as the crosslinking agent. Via orthogonal experiment, the three factors, the ratio of template-functional monomer, template-crosslinking agent and the types of porogen, were investigated, in order to optimize the polymerization conditions. According to the orthogonal experiment, the type of porogen was the most important factor, the ratio of template-functional monomer and ratio of template-crosslinking agent took the subordinate place; when the polymerization conditions were acetonitrile used as porogen, the ratio between ETP-NOBE-EGDMA was 1:2:20, the imprinting effect of ETP-MIPs was the best. The Scatchard analysis indicated that there were two classes of binding sites in ETP-MIPs; the equilibrium dissociation constant (Kd) and apparent maximum binding capacity (Qmax) of high affinity sites and low affinity sites were calculated,respectively were Kd1=1.608 μg/mL, Qmax1=1.101 μg/mg; Kd2=0.109 μg/mL, Qmax2=0.172 μg/mg.

Key words: Molecularly imprinted polymers, Surface molecular imprinting technology, Orthogonal experiment , Ethopabate