食品科学 ›› 2017, Vol. 38 ›› Issue (23): 313-317.doi: 10.7506/spkx1002-6630-201723049

• 专题论述 • 上一篇    

基于人工模拟抗体的酶联检测技术在食品安全检测中的应用

何彦平1,2,金 涌1,3,陈 达2,邢仕歌1,王燕飞1,3,薛 强1,3,齐小花1,3,邹明强1,3,*   

  1. 1.中国检验检疫科学研究院,北京 100123;2.天津大学精密仪器与光电子工程学院,天津 300072;3.中检国研(北京)科技有限公司,北京 100123
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家重点研发计划重点专项(2016YFF0203802);“十二五”国家科技支撑计划项目(2015BAK44B01);国家自然科学基金青年科学基金项目(21305101);国家重大科学仪器设备开发专项(2012YQ0901970501)

Application of Artificial Antibody in Enzyme-Linked Assay for Food Safety Detection

HE Yanping1,2, JIN Yong1,3, CHEN Da2, XING Shige1, WANG Yanfei1,3, XUE Qiang1,3, QI Xiaohua1,3, ZOU Mingqiang1,3,*   

  1. 1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China; 2. School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China; 3. Zhong Jian Guo Yan (Beijing) Technology Co. Ltd., Beijing 100123, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 广义人工模拟抗体包括适配体、重组受体、分子印迹聚合物及多肽。人工模拟抗体与传统抗体相比优势显 著,其具有易实现工业化生产、成本低、保存方便、可反复使用等特点。酶联检测技术是人工模拟抗体在食品安全 检测中应用最为广泛的技术。本文综述了人工模拟抗体的特点以及基于人工模拟抗体的酶联检测技术在食品安全检 测中的应用。

关键词: 适配体, 重组受体, 分子印迹聚合物, 多肽, 人工模拟抗体, 酶联检测技术, 食品安全检测

Abstract: Generalized artificial antibodies include aptamers, recombinant receptors, molecularly imprinted polymers and polypeptides. Artificial antibodies are significantly advantageous over conventional antibodies in terms of their ease of industrial production, low cost, ease of storage and reutilizability. In food safety detection, the most widely used technique is enzyme-linked assay based on artificial antibody. This paper reviews the benefits and drawbacks of applying aptamers, receptor, molecularly imprinted polymers and polypeptides in enzyme-linked assay, as well as the prospect of artificial antibody in enzyme-linked assay for food safety detection.

Key words: aptamer, recombinant receptor, molecularly imprinted polymer, polypeptide, artificial antibody, enzyme-linked assay, food safety detection

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