基于无标记金纳米簇的新型荧光生物传感器在赭曲霉毒素A快速检测中的应用

doi:10.7506/spkx1002-6630-20200212-108

食品科学 ›› 2021, Vol. 42 ›› Issue (4): 263-270.doi: 10.7506/spkx1002-6630-20200212-108

基于无标记金纳米簇的新型荧光生物传感器在赭曲霉毒素A快速检测中的应用

孔德莉，罗思，彭瑞晨，刘嘉睿，文茜

（中南林业科技大学食品科学与工程学院，湖南?长沙 410004）

出版日期:2021-02-25 发布日期:2021-02-25
基金资助:
国家自然科学基金青年科学基金项目（21505040）；湖南省自然科学基金青年项目（2019JJ51004）；中南林业科技大学人才引进科研启动项目（2017YJ016）

Application of a Novel Fluorescent Biosensor Based on Unlabeled Gold Nanoclusters in Rapid Detection of Ochratoxin A

KONG Deli, LUO Si, PENG Ruichen, LIU Jiarui, WEN Qian

(College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China)

Online:2021-02-25 Published:2021-02-25

摘要/Abstract

摘要： 基于快速合成无标记核酸适配体（aptamer，Apt）模板金纳米簇（gold nanocluster，AuNCs），并以核酸Apt部分互补的单链DNA修饰的金纳米颗粒（gold nanoparticles，AuNPs）结合，构建新型荧光复合纳米生物传感器。利用检测靶标与核酸Apt之间更强结合力，使已荧光猝灭的Apt-AuNCs@cDNA-AuNPs复合纳米传感器发生荧光共振能量转移，最终体系荧光强度值恢复的特性，用于快速准确检测赭曲霉毒素A（ochratoxin A，OTA）。结果表明，OTA质量浓度在0.01～2.5 ng/mL之间，荧光强度对应峰值（FI）与OTA质量浓度（C）呈现良好的线性关系，回归方程分别为FI=689.84lgC（OTA）＋8 315.31；检出限为0.025 ng/mL（信噪比为3）。该荧光生物传感器具有合成及操作简单、灵敏度高、选择性强、稳定性好及检测下限低的特点，预期在食品中相关有害因子的安全检测等提供一种新的思路和平台。

关键词: 核酸适配体；金纳米簇；金纳米颗粒；毒素；荧光共振能量转移；赭曲霉毒素A

Abstract: In this study, gold nanoclusters templated with unlabeled nucleic acid aptamers were rapidly synthesized for use in combination with gold nanoparticles modified with single-stranded DNA that is partially complementary to the DNA strands of the aptamer in a novel fluorescent composite nano-biosensor for rapid detection of ochratoxin A. The stronger binding force between the target and aptamer brought about fluorescence resonance energy transfer in the Apt-AuNCs@cDNA-AuNPs sensor whose fluorescence had been quenched and consequently recovery of the lost fluorescence intensity, thereby allowing the rapid and accurate detection of ochratoxin A (OTA). The results showed that the peak value of fluorescence intensity (FI) displayed a good linear relationship with the concentration (C) of ochratoxin A within the range from 0.01 to 2.5 ng/mL, which was fitted as FI = 689.84lgC + 8 315.31, with the limit of detection (LOD) of 0.025 ng/mL at signal-to-noise ratio of 3. This new fluorescent composite biosensor has the characteristics of simple synthesis, easy operation, high sensitivity, strong selectivity, good stability and a low LOD, which is expected to provide a new idea and platform for the detection of food safety hazard factors.

Key words: aptamer; gold nanoclusters; gold nanoparticles; toxin; fluorescence resonance energy transfer; ochratoxin A

中图分类号:

TS201.6

孔德莉，罗思，彭瑞晨，刘嘉睿，文茜. 基于无标记金纳米簇的新型荧光生物传感器在赭曲霉毒素A快速检测中的应用[J]. 食品科学, 2021, 42(4): 263-270.

KONG Deli, LUO Si, PENG Ruichen, LIU Jiarui, WEN Qian. Application of a Novel Fluorescent Biosensor Based on Unlabeled Gold Nanoclusters in Rapid Detection of Ochratoxin A[J]. FOOD SCIENCE, 2021, 42(4): 263-270.

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基于无标记金纳米簇的新型荧光生物传感器在赭曲霉毒素A快速检测中的应用

Application of a Novel Fluorescent Biosensor Based on Unlabeled Gold Nanoclusters in Rapid Detection of Ochratoxin A

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