食品科学 ›› 2021, Vol. 42 ›› Issue (4): 254-262.doi: 10.7506/spkx1002-6630-20200119-248

• 成分分析 • 上一篇    下一篇

超高效液相色谱-紫外检测法测定婴幼儿配方乳粉中的核苷酸含量

王象欣,单艺,魏雪冬,李文斌,鄂来明,姜毓君   

  1. (1.东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江?哈尔滨 150030;2.东北农业大学 黑龙江省绿色食品科学研究院,黑龙江?哈尔滨 150028;3.国家乳制品质量监督检验中心,黑龙江?哈尔滨 150028;4.天津迪科马实验设备有限公司,天津 300402)
  • 出版日期:2021-02-25 发布日期:2021-02-25

Determination of Nucleotides in Infant Formula by Ultra-high Performance Liquid Chromatography

WANG Xiangxin, SHAN Yi, WEI Xuedong, LI Wenbin, E Laiming, JIANG Yujun   

  1. (1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin 150028, China; 3. National Dairy Quality Supervision and Inspection Center, Harbin 150028, China;4. Dikma Technologies Inc., Tianjin 300402, China)
  • Online:2021-02-25 Published:2021-02-25

摘要: 采用磺酸甜菜碱型两性离子亲水相互作用色谱法,建立婴幼儿配方乳粉中5 种核苷酸(5’-胞嘧啶核苷酸、5’-尿嘧啶核苷酸、5’-腺嘌呤核苷酸、5’-次黄嘌呤核苷酸、5’-鸟嘌呤核苷酸)的测定方法。使用二维固相萃取模式,弱阴离子交换和强阳离子交换,对样品中的核苷酸进行提取,紫外检测器采集数据;用0.001%甲酸-乙腈溶液和pH 5的10 mmol/L磷酸二氢钠溶液作为流动相,等度洗脱。5 种核苷酸在其线性范围内线性关系良好,定量限在0.10~0.15 mg/100 g范围内,方法回收率均在95%以上,精密度在10%以内。该方法快速、灵敏、准确,适合各类婴幼儿配方乳粉中核苷酸的测定。

关键词: 核苷酸;磺酸甜菜碱型两性离子亲水相互作用色谱法;二维固相萃取;超高效液相色谱;婴幼儿配方乳粉

Abstract: A method for the quantitation of nucleotides (cytidine 5’-monophosphate, uridine 5’-monophosphate, adenosine 5’-monophosphate, inosine 5’-monophosphate, and guanosine 5’-monophosphate) in infant formula was developed by sulfoalkyl betaine-based zwitterionic hydrophilic interaction liquid chromatography. The nucleotides in samples were extracted by two-dimensional solid-phase extraction with weak anion exchange and strong cation exchange, data acquisition was achieved using a UV detector. Chromatographic separation was performed by isocratic elution with a mobile phase consisting of 0.001% formic acid-acetonitrile and 10 mmol/L sodium dihydrogen phosphate solution at pH 5. Under the optimized conditions, good linearity was observed for these nucleotides, with a limit of quantification in the range of 0.10 to 0.15 mg/100 g. The method recovery was above 95% and the precision (expressed as relative standard deviation, RSD) was within 10%. This method was fast, sensitive and accurate and could be applied for the determination of nucleotides in infant formula samples.

Key words: nucleotides; sulfoalkylbetaine-based zwitterionic hydrophilic interaction liquid chromatography; two-dimensional solid-phase extraction; ultra-high performance liquid chromatography; infant formula

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