食品科学 ›› 2018, Vol. 39 ›› Issue (22): 319-327.doi: 10.7506/spkx1002-6630-201822048

• 安全检测 • 上一篇    下一篇

磺胺嘧啶分子印迹电化学传感器的制备及其快速检测食品中磺胺嘧啶药物残留

赵玲钰,秦思楠,高林,高文惠*   

  1. (河北科技大学生物科学与工程学院,河北省发酵工程技术研究中心,河北?石家庄 050000)
  • 出版日期:2018-11-25 发布日期:2018-11-21
  • 基金资助:
    河北省科技计划项目(16275503D);石家庄市科学技术研究与发展计划项目(181490372A)

Preparation and Application of Molecularly Imprinted Electrochemical Sensor for Rapid Detection of Sulfadiazine Residues in Foods

ZHAO Lingyu, QIN Sinan, GAO Lin, GAO Wenhui*   

  1. (Center for Fermentation Engineering of Hebei Province, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050000, China)
  • Online:2018-11-25 Published:2018-11-21

摘要: 以磺胺嘧啶为模板分子,邻氨基苯酚为功能单体,采用紫外光谱法优化二者比例,以羧基化多壁碳纳米管和纳米金为修饰材料,用滴涂法修饰玻碳电极,在高氯酸-高氯酸钠溶液(pH?5.5)中电聚合形成邻氨基苯酚聚合膜,制备磺胺嘧啶分子印迹电化学传感器。实验选用含0.5?mol/L?KCl及5?mmol/L?K3[Fe(CN)6]-K4[Fe(CN)6]的水溶液为表征溶液,采用循环伏安法和差分脉冲伏安法研究传感器的电化学响应特性,优化制备条件,研究印迹传感器对模板分子及其结构类似物的选择性响应性能,并将该传感器应用于食品中磺胺嘧啶药物残留的快速检测。在最佳条件下,磺胺嘧啶浓度在1.0×10-8~2.0×10-6?mol/L范围内线性关系良好,检出限为3.3×10-9?mol/L,样品加标平均回收率在83.50%~97.80%之间,相对标准偏差不大于4.0%。该传感器制作成本低、操作简便快速、检测灵敏度高、抗干扰能力强、稳定性好,具有良好的应用前景。

关键词: 磺胺嘧啶, 电极修饰材料, 分子印迹聚合物, 电化学传感器, 紫外光谱法

Abstract: A molecularly imprinted electrochemical sensor for the detection of sulfadiazine was prepared using sulfadiazine as a template molecule and o-aminophenol as a functional monomer at an optimized ratio as determined by UV spectroscopy. The glassy carbon electrode was modified by drop casting method with carbonylated multi-walled carbon nanotubes and nano-gold sol. Poly (o-aminophenol) membrane was formed by electropolymerization in perchloric acid-sodium perchlorate solution (pH 5.5). In the experiment, an aqueous solution containing 0.5 mol/L KCl and 5 mmol/L K3[Fe(CN)6] was used for the characterization of the sensor. The response characteristics of the electrochemical sensor were evaluated by cyclic voltammetry and differential pulse voltammetry. The preparation conditions were optimized. The selective response of the sensor to the template molecule and its analogues was investigated. It was applied to the rapid detection of sulfadiazine residues in foods. The results showed that under the optimum conditions, good linearity was observed in the concentration range of 1.0 × 10-8–2.0 × 10-6 mol/L. The limit of detection (LOD) was 3.3 × 10-9 mol/L, and the average recoveries from spiked samples were between 83.50% and 97.80%, with relative standard deviations (RSDs) less than or equal to 4.0%. The sensor is very promising due to its low cost, easy and rapid operation, high sensitivity, strong interference resistance and good stability.

Key words: sulfadiazine, electrode modification material, molecularly imprinted polymer, electrochemical sensor, UV spectroscopy

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