表没食子儿茶素没食子酸酯对抗肿瘤所致心肌损伤的保护作用

doi:10.7506/spkx1002-6630-201611031

食品科学

表没食子儿茶素没食子酸酯对抗肿瘤所致心肌损伤的保护作用

宁佳鸣1，张妍淞1，姚于飞2，王喆1，李露1，陈家俊1，李文娟1,*

1.南昌大学食品科学与技术国家重点实验室，江西南昌 330047；
2.中国人民解放军第九四医院呼吸科，江西南昌 330000

出版日期:2016-06-15 发布日期:2016-06-27

Protective Effect of Epigallocatechin-3-gallate against Antitumor-Induced Myocardial Injury

NING Jiaming1, ZHANG Yansong1, YAO Yufei2, WANG Zhe1, LI Lu1, CHEN Jiajun1, LI Wenjuan1,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. Pneumology Department of China People’s Liberation Army No.94 Hospital, Nanchang 330000, China

Online:2016-06-15 Published:2016-06-27

摘要/Abstract

摘要：

目的：观察表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）的抗肿瘤作用，研究EGCG对抗肿瘤药物阿霉素（adriamycin，ADR）所致小鼠心肌损伤的保护作用及其机制。方法：建立S180小鼠实体瘤模型，随机分为4 组：对照组、ADR组、EGCG组、EGCG＋ADR组。分离肿瘤与心肌组织，分别测定组织内乳酸脱氢酶（lactate dehydrogenase，LDH）、肌酸激酶（creatine kinase，CK)和锰超氧化物歧化酶（manganese superoxidedismutase，MnSOD）的活性；流式细胞仪检测细胞凋亡、活性氧（reactive oxygen species，ROS）及线粒体膜电位。结果：与对照组相比，EGCG和ADR能显著增加肿瘤细胞凋亡作用，且ADR＋EGCG作用更强。与ADR组相比，EGCG＋ADR可显著降低心肌组织中ADR引起的LDH和CK活性的增加。同时，EGCG＋ADR中可显著增加心肌组织的抗氧化酶MnSOD活性，减少ROS的生成，增加线粒体膜电位。结论：EGCG具有抗肿瘤作用，且与ADR合用可显著增强ADR抗肿瘤作用。同时可通过提高线粒体的抗氧化防御、削弱氧化应激、稳定线粒体膜电位，对ADR所致心肌损伤发挥保护作用。

关键词: 肿瘤, 阿霉素, 活性氧, 表没食子儿茶素没食子酸酯

Abstract:

Objective: To examined the antitumor effect of epigallocatechin-3-gallate (EGCG), and to investigate the
protective effect and potential mechanism of EGCG against myocardial injury triggered by the anticancer drug adriamycin
(ADR). Methods: A S180 tumor-bearing mice model was established and the mice were randomly divided into four
groups: control, ADR, EGCG and EGCG + ADR groups. Tumor and myocardial tissues were taken for the measurement
of lactate dehydrogenase (LDH), creatine kinase (CK) and Mn-superoxide dismutase (Mn-SOD) activities using detection
kits according to the manufacturer’s instructions. Meanwhile, apoptosis, reactive oxygen species (ROS) and mitochondrial
membrane potential level were measured by flow cytometry. Results: Compared with the control group, both EGCG and
ADR could significantly increase apoptosis of tumor cells. The combination of EGCG and ADR was superior to either
treatment alone. Moreover, the combined regimen could significantly reduce LDH and CK activities in myocardial tissue.
Compared with ADR alone, its combination with EGCG could significantly increase Mn-SOD activity in myocardial tissue,
reduce the production of ROS and ameliorate the loss of mitochondrial membrane potential. Conclusion: EGCG itself had
anti-tumor effect, and could synergize with ADR. In addition, EGCG had protective effect against myocardial damage
caused by ADR through improving the mitochondrial antioxidant defense capacity, ameliorating oxidative stress and
maintaining △Ψm homeostasis.

Key words: tumor, adriamycin (ADR), reactive oxygen species (ROS), epigallocatechin-3-gallate (EGCG)

中图分类号:

Q946.841

宁佳鸣，张妍淞，姚于飞，王喆，李露，陈家俊，李文娟. 表没食子儿茶素没食子酸酯对抗肿瘤所致心肌损伤的保护作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201611031.

NING Jiaming, ZHANG Yansong, YAO Yufei, WANG Zhe, LI Lu, CHEN Jiajun, LI Wenjuan. Protective Effect of Epigallocatechin-3-gallate against Antitumor-Induced Myocardial Injury[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201611031.

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表没食子儿茶素没食子酸酯对抗肿瘤所致心肌损伤的保护作用

Protective Effect of Epigallocatechin-3-gallate against Antitumor-Induced Myocardial Injury

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