食品科学 ›› 2012, Vol. 33 ›› Issue (2): 154-158.doi: 10.7506/spkx1002-6630-201202033

• 工艺技术 • 上一篇    下一篇

越桔叶超氧化物歧化酶超声波提取工艺的响应面优化

文连奎,贺 阳   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2017-02-16 修回日期:2017-02-16 出版日期:2012-01-25 发布日期:2012-01-16

Optimization of Ultrasonic Extraction Process for Superoxide Dismutase from Bilberry Leaves by Response Surface Methodology

WEN Lian-kui,HE Yang   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2017-02-16 Revised:2017-02-16 Online:2012-01-25 Published:2012-01-16

摘要: 以越橘叶为原料提取超氧化物歧化酶。在单因素试验基础上,选取超声功率、超声时间、液料比3个变量,进行响应面设计,从而对提取条件进行优化。结果表明超声波提取越橘叶超氧化物歧化酶的最佳提取工艺参数为超声功率780W、超声时间5.8min、液料比2.4:1,越橘叶超氧化物歧化酶最终浸提酶比活力为1999.28U/mL。

关键词: 越桔叶, 超氧化物歧化酶, 超声波提取, 响应面

Abstract: The purpose of the present study was to optimize the ultrasonic-assisted extraction of superoxide dismutase (SOD) from bilberry leaves. Process variables such as ultrasonic power, ultrasonic treatment time and material/liquid ratio were optimized using response surface methodology on the basis of one-factor-at-a-time experiments. The results indicated that the optimal extraction conditions were ultrasonic power of 780 W, ultrasonic treatment time of 5.8 min and material/liquid ratio of 1:2.4. Under these conditions, the specific activity of SOD from bilberry leaves was 1999.28 U/mL.

Key words: bilberry leaves, superoxide dismutase, ultrasonic extraction, response surface

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