• 工艺技术 •

### 超高压协同碱性蛋白酶制备玉米黄粉ACE抑制肽

1. （中国农业科学院农产品加工研究所，农业部农产品加工综合性重点实验室，北京 100193）
• 出版日期:2020-02-25 发布日期:2020-03-02
• 基金资助:
国家自然科学基金面上项目（31871730）；农业部引进国际先进农业科学技术计划（948计划）项目（2016-X31）； 中国农业科学院协同创新工程项目（CAAS-XTCX 2016005）；农业部大宗粮食加工重点实验室开放基金项目（DZLS201707）

### Preparation of Angiotensin Converting Enzyme Inhibitory Peptides from Corn Gluten Meal by Ultra-high Pressure-Assisted Alcalase Hydrolysis

LIU Ziyi, GU Fengying, WANG Bolun, ZHANG Fan, LIU Hao, WANG Feng

1. (Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
• Online:2020-02-25 Published:2020-03-02

Abstract: An ultra-high pressure (UHP)-assisted alcalase hydrolysis process was established to prepare angiotensin converting enzyme (ACE) inhibitory peptides for corn gluten meal (CGM) in order to simultaneously increase the ACE inhibitory activity of CGM hydrolysate and the hydrolysis efficiency. A comparative analysis of the effect of UHP-assisted enzymatic hydrolysis, enzymatic hydrolysis after UHP pretreatment and enzymatic hydrolysis under atmospheric pressure was made on the inhibition rate of ACE. In the UHP-assisted enzymatic hydrolysis method, the operating parameters substrate concentration, time, pressure, pH and temperature were evaluated for their effects on the inhibition rate of ACE. These preparation conditions were optimized by response surface methodology (RSM). The ACE inhibition rate of the hydrolysate obtained by UHP-assisted hydrolysis (for 15 min) was increased by 55% compared with that obtained under atmospheric pressure, and 30% higher than that obtained with UHP pretreatment. pH was found to be the main factor affecting the ACE inhibition rate, followed by pressure, substrate concentration and time. The effect of temperature was not obvious. The results indicated that the ACE inhibition rate was up to 74.30% under the optimized conditions: substrate concentration 4.78%, high pressure holding time 14 min, pressure 372 MPa, and pH 8.4. UHP-assisted alcalase hydrolysis could not only increase the ACE inhibition rate but also shorten the hydrolysis time to within 15 min, which would provide a basis for the green and efficient preparation of corn protein-derived functional peptides.