食品科学 ›› 2011, Vol. 32 ›› Issue (9): 213-216.doi: 10.7506/spkx1002-6630-201109048

• 生物工程 • 上一篇    下一篇

酿酒活性干酵母生产工艺优化及干燥剂的选择

张建峰,耿宏伟,王丕武*   

  1. 吉林农业大学生命科学学院
  • 出版日期:2011-05-15 发布日期:2011-04-11
  • 基金资助:
    吉林省教育厅“十一五”科研规划项目(吉教科2009-363);吉林农业大学科研启动基金项目(吉农215-00081)

Process Optimization and Protective Agents for Alcohol Active Dry Yeast

ZHANG Jian-feng,GENG Hong-wei,WANG Pi-wu*   

  1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 对酿酒活性干酵母(AADY)生产工艺进行优化及选定生产用保护剂。酿酒酵母体积分数1%~3%的接种量接于发酵罐,28℃、200r/min,通气量10NL/min,培养40h;流化床干燥器进风温度90℃;司班-60 2%+吐温-80 2%的组合作为生产用保护剂,AADY活菌率可高达77.89%;海藻糖5%+司班-60 2%+吐温-80 2%的组合作为高端生产用保护剂或者用于保藏重要菌种,AADY活菌率可达(83.52±1.87)%。

关键词: 酿酒活性干酵母, 干燥保护剂, 生产工艺

Abstract: Through a series of experiments, the process for producing alcohol active dry yeast (AADY) was optimized and the optimum protective agent was selected. Saccharomyces cerevisiae was inoculated at 1%-3% (V/V) into a fermentor for the optimized 40 h fermentation at 28 ℃ and 10 NL/min air flow with shaking at 200 r/min. The inlet temperature of fluidized bed was set at 90 ℃. The protective agent used for ordinary AADY was a combination of 2% Span-60 and 2% Tween-80 and the percentage of living AADY cells was up to 77.89%. The combination of 5% trehalose, 2% Span-60 and 2% Tween-80 could be used for the protection of high-quality AADY and strain conservation and keep (83.52 ± 1.87) % of AADY cells alive.

Key words: alcohol active dry yeast (AADY), drying protective agent, process

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