江蓠藻渣膳食纤维制备工艺

doi:10.7506/spkx1002-6630-201124007

食品科学 ›› 2011, Vol. 32 ›› Issue (24): 31-35.doi: 10.7506/spkx1002-6630-201124007

江蓠藻渣膳食纤维制备工艺

戚勃1，杨贤庆1,*，李来好1，赵永强1,2，陈胜军1，岑剑伟1

1.中国水产科学研究院南海水产研究所，国家水产品加工技术研发中心 2.中国海洋大学食品科学与工程学院

出版日期:2011-12-25 发布日期:2011-12-29
基金资助:
农业部公益性行业(农业)科研专项(200903030-C)；国家海洋局海洋公益性行业科研专项(201005020-7)；广东省科技计划项目(2010B020201015)；广东省海洋渔业科技推广专项(A20108I02；A200901I02)

Optimization of Enzymatic Preparation of Dietary Fiber from Gracilaria Waste from Agar Production

QI Bo1，YANG Xian-qing1,*，LI Lai-hao1，ZHAO Yong-qiang1,2，CHEN Sheng-jun1，CEN Jian-wei1

(1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , National R&D Center for Aquatic Product Processing, Guangzhou 510300, China；2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)

Online:2011-12-25 Published:2011-12-29

摘要/Abstract

摘要： 目的：研究从江蓠藻渣中制备膳食纤维的工艺。方法：采用梯度离心法脱除助滤剂，得到藻渣粗纤维；再以可溶性膳食纤维得率为指标，利用复合植物水解酶对其进行酶解改性，在单因素试验基础上，采用正交试验对酶解工艺条件进行优化。结果：梯度离心条件分别为2500r/min、10min，3500r/min、5min，可得到纯净的藻渣粗纤维，回收率为25.4%，同时回收得到60.2%的助滤剂；藻渣粗纤维酶解改性的最佳工艺条件为料液比1:30(g/mL)、加酶量20FBG/g、pH4.5、酶解时间2.5h、酶解温度55℃，酶解改性后总膳食纤维得率为20.34%(相对于藻渣，干质量计)，膨胀力10.25mL/g，持水力541.6%。结论：梯度离心结合复合植物水解酶制备江蓠藻渣膳食纤维可行，可为江蓠藻渣的高值化利用提供理论基础。

关键词: 江蓠, 藻渣, 膳食纤维

Abstract: Objective: To optimize the enzymatic preparation of dietary fiber from Gracilaria waste from agar production. Methods: Crude fiber was obtained from the waste by gradient centrifugation for the removal of filter-aid agents and hydrolyzed by viscozyme; after completion of the hydrolysis, centrifugation was carried out to collect the precipitate as dietary fiber. Crude fiber hydrolysis was optimized using orthogonal array design based on one-factor-at-a-time experiments. Results: crude fiber was obtained with a recovery of 25.4% after gradient centrifugation at 2500 r/min for 10 min and then at 3500 r/min for 5 min and meanwhile, filter-aid agents were recovered by 60.2%. The optimal conditions for crude fiber hydrolysis were 1:30 material/liquid ratio (g/mL), pH 4.5, 20 FBG/g enzyme dosage, 55 ℃, and 2.5 h. Under these conditions, the yield of dietary fiber was 20.34% (relative to Gracilaria waste based on dry matter). The swelling power and water-holding capacity of the obtained product were 10.25 mL/g and 541.6% respectively. Conclusion: Gradient centrifugation and then viscozyme hydrolysis is feasible for preparing dietary fiber from Gracilaria waste from agar production.

Key words: Gracilaria, residue, dietary fiber

中图分类号:

TS201.4

戚勃，杨贤庆，李来好，赵永强，陈胜军，岑剑伟. 江蓠藻渣膳食纤维制备工艺[J]. 食品科学, 2011, 32(24): 31-35.

QI Bo，YANG Xian-qing，LI Lai-hao，ZHAO Yong-qiang，CHEN Sheng-jun，CEN Jian-wei. Optimization of Enzymatic Preparation of Dietary Fiber from Gracilaria Waste from Agar Production[J]. FOOD SCIENCE, 2011, 32(24): 31-35.

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江蓠藻渣膳食纤维制备工艺

Optimization of Enzymatic Preparation of Dietary Fiber from Gracilaria Waste from Agar Production

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