食品科学 ›› 2012, Vol. 33 ›› Issue (6): 69-73.doi: 10.7506/spkx1002-6630-201206015

• 工艺技术 • 上一篇    下一篇

大蕉淀粉的复合酶法改性工艺优化

黄守耀1,夏 雨1,杜 冰1,原敏敏1,鲁旺旺1,白永亮1,徐 勇1,2,杨公明1,*   

  1. 1.华南农业大学食品学院 2.广东省食品工业研究所
  • 出版日期:2012-03-25 发布日期:2012-03-03
  • 基金资助:
    粤港关键领域重点突破项目(2008A024200004);东莞市科技计划项目(200910810100600); 东莞市2009 年科技计划高校科研机构重点项目(200910810110);广东省农业厅2009 年省级良种培育与引进专项

Optimization of Double-Enzymatic Modification of Plantain Starch

HUANG Shou-yao1,XIA Yu1,DU Bing1,YUAN Min-min1,LU Wang-wang1, BAI Yong-liang1,XU Yong1,2,YANG Gong-ming1,*   

  1. (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Guangdong Food Industry Institute, Guangzhou 510308, China)
  • Online:2012-03-25 Published:2012-03-03

摘要: 利用复合酶对大蕉淀粉进行改性研究,在单因素试验基础上进行二次正交旋转回归设计优化大蕉淀粉复合酶法改性的最佳工艺条件,并对改性前后大蕉淀粉颗粒形态进行观察比较。结果表明:复合酶法改性大蕉淀粉的最佳工艺条件为淀粉乳质量分数5%、酶用量25U/mL、60℃酶解24h。在此条件下,大蕉淀粉的水解率为16.17%,与模型预测值基本一致。扫描电镜显示,改性淀粉颗粒出现孔洞,形貌更加圆滑,粒径有所减小,分布较为均匀,可有效提高大蕉淀粉加工特性和改善产品口感。

关键词: 大蕉淀粉, 复合酶解, 酶法改性, 微观结构

Abstract: The compound enzyme was used for the modification of plantain starch in this article. On the basis of single factor tests, orthogonal rotation combination design was used to optimize modification process of plantain starch. The microscopic shape of starch was observed. The results indicated that the optimal conditions for plantain starch modification by compound enzyme were starch milk concentration 5%, compound enzyme concentration 25 U/mL, enzymatic hydrolysis time of 24 h and hydrolysis temperature of 60 ℃. Under the optimal hydrolysis conditions, the hydrolysis rate of plantain starch was 16.17%, which was consistent with the predicted value. The scanning electron microscope revealed a lot of keyholes, smoother shape, reduced and evenly distributed particle size in modified plantain starch. Meanwhile, the quality and taste of modified plantain starch were significantly improved.

Key words: plantain starch, compound enzymatic hydrolysis, enzymatic modification, microscopic structure

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