关键词: 姜黄素, 幽门螺杆菌, 脲酶, 人胃上皮细胞

Abstract: The inhibition of curcumin on Helicobacter pylori (Hp) and jack bean urease activity was studied. The effect of curcumin on normal GES-1 cells and Hp induced GES-1 injury was also researched. The results of bacteriostatic experiments showed that curcumin significantly inhibited Hp proliferation, with the MIC of 200 μM. Moreover, curcumin showed obvious inhibitory effect on jack bean urease activity, with the IC50 value of 1.735 mM. Curcumin could inhibit human gastric epithelial GES-1 cells in a dose- and time-dependent manner. After infecting GES-1 cells with Hp for 24 h, the GES-1 cells injury model was successfully established to study the effect of curcumin on injured GES-1 cells. After treatment with curcumin at the concentration of 68μM for 12 h, the injury cell model morphology was slightly restored , the survival rate of GES-1 cells slightly increased. But the survival rate significantly decreased after treatment with curcumin at the concentration of 136 μM or 680 μM. Thus, inhibition of curcumin on Hp and jack bean urease could not obviously help to repair Hp induced GES-1 cells damage. It may be helpful to reduce Hp induced GES-1 cells damage with the synergistic effects between curcumin and other bioactive ingredients with promoting effect on GES-1 cells proliferation.

Key words: Curcumin, Helicobacter pylori, Urease, GES-1 cell