水杨酸处理诱导采后甜瓜ASA-GSH循环代谢清除过氧化氢的作用及机制

摘要/Abstract

摘要： 【目的】研究AsA-GSH循环在水杨酸处理诱导的过量过氧化氢清除过程中的作用。【方法】用 4 mmol·L-1水杨酸浸泡“玉金香”厚皮甜瓜10 min，测定处理果实细胞膜的完整性，分析活性氧的积累水平、超氧化物歧化酶和过氧化氢酶活性，以及AsA-GSH循环作用酶活性以及产物和底物含量。【结果】水杨酸处理降低了果实细胞膜透率和丙二醛含量，第6d处理组细胞膜透率低于对照51%，第10d 处理组MDA含量低于对照14.6%。SA处理显著提高了果实O2-的产生速率和H2O2含量，其中处理后第2d O2-的产生速率高于同期对照的1.9倍，第6d H2O2含量高出对照果实29.7%。SA提高了果实超氧化物歧化酶活性，但抑制了过氧化氢酶活性。SA处理提高了果实抗坏血酸过氧化物酶、单脱氢抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活性，增加了抗坏血酸和氧化型谷胱甘肽的含量，降低了脱氢抗坏血酸和还原型谷胱甘肽的含量。【结论】SA处理诱导了厚皮甜瓜果实的氧爆，维持了果实细胞膜的稳定性，由SA诱导产生的过量H2O2主要AsA-GSH循环清除。

关键词: 厚皮甜瓜, 水杨酸, 活性氧, AsA-GSH循环

Abstract: Abstract: 【Objective】An experiment was conducted to assay AsA-GSH cycle scavenging the H2O2 in muskmelons induced by salicylic acid (SA) during storage. 【Method】The muskmelons (cv. Yujinxiang) were used as experimental material, soaked in 4 mmol L-1 SA for 10 min, and stored at ambient temperature. The cell membrane integrity and the accumulation level of ROS were measured in treat fruit. The activity of superoxide dismutase and catalase was determined, also with the activity of relative enzymes and the contents of substrate and product of AsA-GSH cycle. 【Results】SA treatment maintained cell membrane integrity by reducing the membrane permeability and the malondialdehyde content. After 6 days of treatment, the cell membranes permeability of treated fruit was 51% lower than that control. The MDA content was 14.6% lower than that the control after 10 days of treatment. SA significantly increased the O2- production rate and H2O2 content in fruit. The O2- production rate was 1.9 times higher in treated fruits than that the control after 2 days of treatment. The H2O2 content was 29.7% higher than that the control after 6 days of treatment. The chemical increased superoxide dismutase activity and inhibited catalase activity. SA increased the activity of ascorbate peroxidase, glutathione reductase, monodehydroascorbate peroxidase and dehydroascorbate reductase. Meanwhile, SA also significantly increased the content of ascorbic acid and oxidized glutathione, and decreased the content of dehydroascorbic acid and reduced glutathione. 【Conclusion】SA treatment promotes oxygen burst in muskmelons and maintains cell membrane integrity..However, a the scavenging of higher level H2O2 mainly depend on AsA-GSH cycle.

Key words: muskmelon, salicylic acid, reactive oxygen species, AsA-GSH cycle

中图分类号:

TS205

杨乾毕阳. 水杨酸处理诱导采后甜瓜ASA-GSH循环代谢清除过氧化氢的作用及机制[J]. 食品科学, 0, (): 0-0.

乾杨. AsA-GSH cycle participates in H2O2 scavenging in muskmelons induced by salicylic acid[J]. FOOD SCIENCE, 0, (): 0-0.

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