食品科学 ›› 2022, Vol. 43 ›› Issue (21): 275-281.doi: 10.7506/spkx1002-6630-20210926-312

• 包装贮运 • 上一篇    

采前氨基寡糖素喷施通过激活苯丙烷途径促进采后厚皮甜瓜愈伤

李宝军,刘志恬,薛素琳,柴秀伟,李志程,毕阳,Dov PRUSKY,张锋岗   

  1. (1.甘肃农业大学食品科学与工程学院,甘肃 兰州 730070;2.以色列农业研究组织农产品采后科学部,以色列 里雄莱锡安 7505101;3.海南正业中农高科股份有限公司,海南 海口 570216)
  • 发布日期:2022-12-12
  • 基金资助:
    公益性行业(农业)科研专项(201303075)

Preharvest Sprays with Amino Oligosaccharide Promote Wound Healing in Harvested Muskmelons by Activating the Phenylpropane Pathway

LI Baojun, LIU Zhitian, XUE Sulin, CHAI Xiuwei, LI Zhicheng, BI Yang, Dov PRUSKY, ZHANG Fenggang   

  1. (1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel; 3. Hainan Zhengye Zhongnong High-tech Co., Ltd., Haikou 570216, China)
  • Published:2022-12-12

摘要: 目的:探讨果实发育期氨基寡糖素多次喷施对采后厚皮甜瓜愈伤的影响。方法:在果实发育的4 个时期(幼果期、膨大前期、膨大后期及成熟期)用2 mL/L氨基寡糖素对‘玛瑙’甜瓜植株和果实进行4 次喷施。评价氨基寡糖素处理对采后果实愈伤期间的质量损失率和病情指数的影响,观察伤口表面封闭层的形成情况,分析相关代谢酶活力。结果:氨基寡糖素喷施有效降低了愈伤期间损伤果实的质量损失率,以及Trichothecium roseum接种损伤果实的病情指数,愈伤第7天时处理组果实的质量损失率和病情指数分别比同期对照组低28.64%和32.00%。氨基寡糖素喷施促进了伤口处聚酚软木脂和木质素的沉积,愈伤第5天时处理组果实伤口处两种物质沉积的细胞层厚度分别高出对照组22.70%和25.07%。喷施处理提高了伤口处的苯丙氨酸解氨酶活力以及总酚、类黄酮、木质素含量。此外,喷施处理还提高了果实伤口处的H2O2含量,增强了过氧化物酶活力。结论:氨基寡糖素采前多次喷施可通过激活采后厚皮甜瓜的苯丙烷代谢,提高H2O2含量及过氧化物酶活力,从而促进果实愈伤。

关键词: 氨基寡糖素;采前喷施;厚皮甜瓜;愈伤;苯丙烷代谢

Abstract: We aimed to study the effects of multiple sprays with amino oligosaccharide (AOS) during fruit development on wound healing in harvested muskmelons. The muskmelon plants and fruits (cv. Manao) were sprayed with AOS at 2 mL/L for four times at the four stages (young fruit stage, early stage of enlargement, late stage of enlargement and mature stage) of fruit development. The effect of AOS sprays on the mass loss and disease index of harvested fruits during wound healing was evaluated. The formation of closing layer was observed on the wounds. Related metabolic enzyme activities were determined. AOS sprays effectively reduced the mass loss and disease index of wounded fruits inoculated with Trichothecium roseum during wound healing. On day 7, the mass loss and disease index of the treated group were 28.64% and 32.00% lower than those of the control group, respectively. The sprays promoted the deposition of suberin polyphenolics and lignin at wound sites, and the cell layer of suberin polyphenolics and lignin deposited was 22.70% and 25.07% thicker than those in the control group on the 5th day of healing. The sprays also increased phenylalanine ammonia lyase activity, and enhanced the contents of total phenols, flavonoids and lignin. In addition, the sprays significantly raised H2O2 content and peroxidase activity at wound sites. Taken together, preharvest multiple sprays with AOS could promote wound healing in harvested muskmelons by activating phenylpropane metabolism, and increasing H2O2 content and peroxidase activity.

Key words: amino oligosaccharide; preharvest sprays; muskmelons; wound healing; phenylpropane metabolism

中图分类号: