链格孢侵染采后甜瓜病程相关蛋白基因表达及酶活性变化规律

摘要/Abstract

摘要： 以伽师瓜和86-1甜瓜为试材，损伤接种链格孢菌，比较两种甜瓜果皮与果肉抗病性差异。甜瓜果实接种链格孢，7±1℃(RH 85%-90%)冷库贮藏，测定病斑直径，观察病斑组织显微结构，荧光定量PCR比较CmGLU、CmCHT1、CmCHT2相对表达量，测定几丁质酶（chitinase，CHT）和β-1,3-葡聚糖酶（β-1,3-glucanase，GLU）活性。结果表明：链格孢侵染两种甜瓜，伽师瓜病斑直径小于86-1甜瓜，果皮小于果肉，30d时，86-1甜瓜果皮和果肉病斑直径是伽师瓜的1.34倍和1.39倍；观察病斑组织显微结构，发现伽师瓜和86-1甜瓜果皮通过细胞壁增厚来抵御链格孢侵染，果肉组织细胞壁抵抗能力较弱，侵染末期果皮与果肉组织细胞壁分层、皱缩，细胞内布满菌丝体和分生孢子，部分果肉细胞解体，无法辨认细胞结构；伽师瓜CHT和GLU基因始终高于86-1甜瓜，果皮高于果肉，CmCHT1相对表达量在侵染初期至侵染中期较高，CmCHT2相对表达量在侵染中期表达量较高，表明病原菌在侵染初期至侵染中期诱导了CHT基因的表达；CmGLU相对表达量在侵染中期至侵染末期表达量较高，表明病原菌在侵染中期至侵染末期诱导了GLU基因的表达；链格孢侵入伽师瓜和86-1甜瓜后，果皮和果肉组织CHT和GLU活性随贮藏时间呈升高-降低的变化趋势，伽师瓜高于86-1甜瓜，果皮高于果肉。表明伽师瓜更强的抵御能力与CHT、GLU基因与酶活性密切相关。

关键词: 甜瓜, 链格孢, 几丁质酶, β-1,3-葡聚糖酶, 基因表达, 酶活性

Abstract: In this study, Alternaria alternata was inoculated to Jiashi melon and 86-1 melon to compare the difference of disease resistance. Two melon cultivars were separately inoculated with A. alternata and subsequent storage at 7 ± 1°C (RH 85% - 90%). Afterwards, lesion size was measured, microstructure of the lesion was observed, the expression levels of CmCht1, CmCht2 and CmGlu genes were investigated by quantitative PCR, and the enzymes activities of chitinase (CHT) and β-1,3-glucanase (GLU) were measured. The results revealed that the lesions of Jiashi melon and 86-1 melon appeared on the 9th day and 6th day. The lesion size in Jiashi melon was smaller than that in 86-1 melon during the whole storage period, and the lesion size of peel was smaller than in pulp. Moreover, lesion size of peel and pulp in 86-1 melon was 1.34 times and 1.39 times greater than that in Jiashi melon on the 30th day. It was also found that the cell structure of the peel and pulp in two melon cultivars were damaged with varying degrees. The cell wall of peel was thickened to resist the infection, however, the pulp was weaker than peel. During the late period, the cell wall of peel and pulp were stratified and shrunk, and filled with hyphae and spores. Moreover, some of the pulp cells were disintegrated and were mostly unable to recognize. The relative expression levels of CHT and GLU genes in Jiashi melon were higher than 86-1 melon during the storage period, and peel was higher than pulp. The relative expression level of CmCHT1 was higher during the early to the middle period, and the relative expression level of CmCHT2 was higher during the middle period, which suggesting that infection with A. alternata stimulated the expression of CHT gene. Moreover, the relative expression levels of CmGLU was higher during the middle to the late period, which suggesting that infection with A. alternata stimulated the expression of GLU gene. After infection with A. alternata, the enzymes activities of CHT and GLU in peel and pulp tissue were significantly increased and decreased with storage time, Jiashi melon was higher than 86-1 melon, and peel was higher than pulp. All these results reveled that Jiashi melon showed stronger disease resistance than 86-1 melon and that was higher in and peel than in pulp, which was closely related with the expression levels and enzymes activities of CHT and GLU.

Key words: muskmelon, Alternaria alternata, chitinase, β-1,3-glucanase, gene expression, enzyme activities

中图分类号:

S652.1

王瑾白羽嘉冯作山朱婉彤曾禹睿慷蒋莉莹. 链格孢侵染采后甜瓜病程相关蛋白基因表达及酶活性变化规律[J]. 食品科学, 0, (): 0-0.

Wang-Jin WANGjin 白羽嘉 feng Wan-Tong ZHU. Study on the gene expression and enzyme activity of the Pathogenesis-related proteins in Postharvest muskmelon infected by Alternaria alternata[J]. FOOD SCIENCE, 0, (): 0-0.

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