食品科学 ›› 2010, Vol. 31 ›› Issue (24): 366-369.doi: 10.7506/spkx1002-6630-201024080

• 分析检测 • 上一篇    下一篇

胶体金试纸条快速检测食品中磺胺二甲基嘧啶残留

林晓丽1,饶 辉2,熊勇华1,陈 媛3,刘文娟3,赖卫华1 ,*   

  1. 1. 南昌大学 食品科学与技术国家重点实验室
    2. 江西省兽药饲料监察所 3. 江西中德生物工程有限公司
  • 收稿日期:2010-08-03 修回日期:2010-11-14 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 赖卫华 E-mail:talktolaiwh@163.com
  • 基金资助:

    教育部“长江学者和创新团队发展计划”资助项目(IRT0540);南昌市重大产业化项目(2009)

Rapid Detection of Sulfadimidine Residue in Food by Colloidal Gold Strips

LIN Xiao-li1,RAO Hui 2,XIONG Yong-hua1,CHEN Yuan3,LIU Wen-juan3,LAI Wei-hua1,*   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ;
    2. Jiangxi Provincial Institute of Veterinary Drug and Feed Control, Nanchang 330029, China;
    3. Jiangxi Zodolabs Biological Engineering Co. Ltd., Nanchang 330029, China
  • Received:2010-08-03 Revised:2010-11-14 Online:2010-12-25 Published:2010-12-29
  • Contact: LAI Wei-hua1 E-mail:talktolaiwh@163.com

摘要:

目的:旨在建立一种快速、简便的针对磺胺二甲基嘧啶残留的检测方法。方法:应用竞争抑制免疫层析原理,研制磺胺二甲基嘧啶胶体金试纸条。结果:该试纸条检测限为10ng/mL、检测时间5min,与其他磺胺类药物交叉反应小,可用于多种食品样本的检测,且样本检测限为100μg/kg。结论:该方法操作简便、灵敏度高且特异性强,可实现对食品样品中磺胺二甲基嘧啶残留的快速检测。

关键词: 磺胺二甲基嘧啶, 胶体金, 试纸条, 快速检测

Abstract:

Objective: To establish a rapid and convenient detection method for sulfadimidine residue in food. Methods: The colloidal gold strip for specific sulfadimidine was developed successfully on the basis of competitive inhibition immunochromatography mechanism. Results: The detection limit of the colloidal gold strip was 10 ng/mL for sulfadimidine within less than 5 min, which also had low cross-reactivity with other sulfonamides. The colloidal gold strip could be used in rapid detection of sulfadimidine residue in many kinds of food samples with detection limit of 100 μg/kg. Conclusion: This method is characteristics of simplicity, high sensitivity and specificity to achieve the rapid detection of sulfadimidine residue in food.

Key words: sulfadimidine, colloidal gold, strip, rapid detection

中图分类号: