食品科学 ›› 2010, Vol. 31 ›› Issue (22): 91-95.doi: 10.7506/spkx1002-6630-201022019

• 工艺技术 • 上一篇    下一篇

响应面优化超声波提取山毛豆抑菌物质条件研究

胡林子1,2,蒋 雨3,马永全2,于 新2,*   

  1. 1.沈阳农业大学食品学院 2.仲恺农业工程学院轻工食品学院 3.四川农业大学食品学院
  • 收稿日期:2010-01-13 修回日期:2010-06-29 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 于 新 E-mail:yuxin1959@yahoo.com.cn

Ultrasound-assisted Extraction of Antimicrobial Substances from Tephrosia vogelii Hook f. Seeds

HU Lin-zi1,2,JIANG Yu3,MA Yong-quan2,YU Xin2,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China;
    2. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    3. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2010-01-13 Revised:2010-06-29 Online:2010-11-25 Published:2010-12-29
  • Contact: YU Xin2,* E-mail:yuxin1959@yahoo.com.cn

摘要:

考察料液比、提取时间、提取溶剂对超声波法提取山毛豆种子中抑菌物质的影响。在单因素试验基础上,利用响应面分析法对山毛豆抑菌物质的提取工艺进行优化。结果得到山毛豆抑菌活性物质的最佳提取工艺条件为液料比12:1(mL/g)、乙醇体积分数55%、超声波提取52min。该条件下质量分数为20%的山毛豆提取液对金黄色葡萄球菌的抑菌圈直径为20.24mm,与理论最优值20.70mm 接近。

关键词: 山毛豆种子, 超声波提取, 抑菌活性, 响应面法, 工艺条件

Abstract:

Ultrasonic wave was applied for extraction of effective component from Tephrosia vogelii Hook f. seeds. Effects of liquid/material ratio, extraction time and ethanol concentration on the antimicrobial effect of the extract were investigated. On the basis of single factor tests, the method of response surface analysis (RSA) with three factors and three levels was adopted. Results showed that the optimum conditions were as follows: liquid-to-material ratio 12:1 mL/g, the ethanol concentration 55% and the ultrasonic processing time 52 min. Under these conditions, the inhibition zone of 20% original concentrated extract for Staphlococcus aureus reached up to 20.24 mm, which was in close agreement of the predicted value(20.70 mm).

Key words: Tephrosia vogelii Hook f. seeds, ultrasound-assisted extraction, antimicrobial activity, response surface methodology, technological conditions

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