食品科学 ›› 2020, Vol. 41 ›› Issue (23): 83-90.doi: 10.7506/spkx1002-6630-20191112-157

• 基础研究 • 上一篇    下一篇

啤酒花中不同比例β-酸同系物的抗氧化及抑菌活性

杨静文,刘泽畅,陈培,杜伟栋,范效兰,石铭,刘玉梅   

  1. (新疆大学化工学院,新疆 乌鲁木齐 830046)
  • 出版日期:2020-12-15 发布日期:2020-12-28
  • 基金资助:
    国家大学生创新训练计划项目(201810755046);国家自然科学基金地区科学基金项目(31660490)

Antioxidant and Antibacterial Activities of β-Acid Homologue Mixtures with Different Ratios from Hops

YANG Jingwen, LIU Zechang, CHEN Pei, DU Weidong, FAN Xiaolan, SHI Ming, LIU Yumei   

  1. (School of Chemical Engineering and Technology, Xinjiang University, ürümqi 830046, China)
  • Online:2020-12-15 Published:2020-12-28

摘要: 啤酒花软树脂中β-酸主要由合蛇麻酮、正蛇麻酮和加蛇麻酮3 种同系物组成,啤酒花的品种不同,上述同系物会存在一定的比例差异。为探究啤酒花中不同比例β-酸同系物的抗氧化及抑菌活性,本实验在分离得到β-酸粗提物的基础上,采用结晶法获取了5 种不同比例β-酸同系物样品,并通过测定黄嘌呤氧化酶活性抑制能力、1,1-二苯基-2-三硝基苯肼自由基和羟自由基清除能力、总抗氧化活性以及抑菌圈直径,对上述样品抗氧化活性和抑菌活性的差异进行比较研究。结果表明:不同比例β-酸同系物样品在抗氧化活性方面存在较大差异,其中合蛇麻酮与正蛇麻酮+加蛇麻酮的质量比为1∶4时,样品的抗氧化活性最强,说明正蛇麻酮+加蛇麻酮的抗氧化活性强于合蛇麻酮。在较高质量浓度(≥40.00 mg/L)时,高比例合蛇麻酮的β-酸样品抑菌活性均较强。除合蛇麻酮与正蛇麻酮+加蛇麻酮的质量比为1∶4的样品外,质量浓度为160.00 mg/L的样品与同质量浓度下阳性对照阿莫西林的抑菌效果相当,甚至更高;高比例正蛇麻酮+加蛇麻酮的β-酸样品对金黄色葡萄球菌的最小抑菌浓度最低,仅为5.00 mg/L,此外,尽管该样品对大肠杆菌的最小抑菌浓度与其余样品均为10.00 mg/L,但其抑菌圈直径更大。

关键词: 啤酒花;软树脂;β-酸同系物;抗氧化活性;抑菌活性

Abstract: Hops soft resin contains three homologues of β-acids: co-, n- and ad-lupulone, and the ratio among these homologues varies depending on hops varieties. In this experiment, five β-acid homologue mixtures were obtained from the crude β-acid from hops by crystallization, and their antioxidant activity in vitro were investigated comparatively by measuring the inhibitory activity against xanthine oxidase, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and OH radical scavenging activity, total antioxidant activity and inhibition zone diameter. The results showed that the antioxidant activity of these β-acid homologue mixtures were significantly different. Among them, the 1:4 mixture of co-lupulone with n-lupulone + ad-lupulone possessed the strongest antioxidant activity, indicating that the antioxidant activity of n-lupulone + ad-lupulone was stronger than that of co-lupulone. At high concentrations (≥ 40.00 mg/L), mixtures with higher proportion of co-lupulone had stronger antibacterial activity. The antibacterial activity of all samples except the 1:4 mixture of co-lupulone with n-lupulone + ad-lupulone at a concentration of 160.00 mg/L was similar to or even stronger than that of amoxicillin. The sample with the highest proportion of n + ad-lupulone showed the lowest minimum inhibitory concentration (5.00 mg/L) against Staphylococcus aureus. In addition, although the minimum inhibitory concentrations of all samples against Escherichia coli were equally 10.00 mg/L, this one exhibited a bigger diameter of inhibition zone.

Key words: hops; soft resin; β-acid homologues; antioxidant activity; antibacterial activity

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