食品科学 ›› 2010, Vol. 31 ›› Issue (22): 457-461.doi: 10.7506/spkx1002-6630-201022103

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采后一氧化氮处理调控番茄果实茉莉酸类物质合成并提高灰霉病抗性

刘零怡1,于萌萌1,郑 杨1,生吉萍1,申 琳1,2,*   

  1. 1.中国农业大学食品科学与营养工程学院
    2.新疆农业大学食品科学与药学学院
  • 收稿日期:2010-05-12 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 申 琳 E-mail:pingshen@cau.edu.cn
  • 基金资助:

    国家自然科学基金项目(30671471;30972065;31071623):“十一五”国家科技支撑计划项目(2006BAD22B07);
    国家公益性行业(农业)科技专项(200803033)

Effect of Postharvest Nitric oxide Treatment on Jasmonates Biosynthesis and Disease Incidence to Botrytis cinerea Pers. in Tomato Fruits

LIU Ling-yi1,YU Meng-meng1,ZHENG Yang1,SHENG Ji-ping1,SHEN Lin1,2,*   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. College of Food Science and Pharmaceutical Science, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2010-05-12 Online:2010-11-25 Published:2010-12-29
  • Contact: SHEN Lin1 E-mail:pingshen@cau.edu.cn

摘要:

探讨在番茄果实抗病性诱导过程中一氧化氮对茉莉酸类物质的调节作用。以中蔬4 号绿熟期番茄果实为材料,对其进行不同浓度一氧化氮处理,检测不同处理对番茄果实抵抗病原菌侵染能力、一氧化氮水平、茉莉酸类物质含量及其关键合成酶活性以及抗病酶苯丙氨酸解氨酶活性的影响。结果表明:不同浓度一氧化氮处理均能提高采后番茄果实抵抗病原菌侵染的能力,低浓度一氧化氮供体硝普钠(0.02mmol/L)表现得更为明显;低浓度硝普钠能够促进JAs 含量及脂氧合酶活性的增加,高浓度处理则表现出抑制作用。

关键词: 番茄果实, 一氧化氮, 茉莉酸类物质, 抗病性

Abstract:

Nitric oxide (NO) and jasmonates (JAs) are important signaling molecules in plant disease resistance. Recent studies have shown the evidences about NO cross talks with jasmonic aicd (JA) pathway. In order to find the relationship between NO and JAs during disease resistance induction, tomato fruits (Lycopersicon esculentum cv. No.4 Zhongshu) were treated with different concentration of NO, then measured the effects on resistance to Botrytis cinerea Pers., NO level, JAs content, the key enzyme activity of JA biosynthesis and phenylalnine ammonialyase activity which is related to disease resistance. Our results showed that treatment with NO could induce disease resistance at all NO levels tested, however, the most obvious induction symptom was recorded in low concentration (0.02 mmol/L). Low concentration treatment increased JAs contents and the key enzyme activity of JA biosynthesis, while inhibited in high concentration treatment.

Key words: tomato fruits, nitric oxide, jasmonates, disease resistance

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