β-氨基丁酸处理对采后桃果实还原势的影响及抗病性的诱导作用

doi:10.7506/spkx1002-6630-20171018-174

食品科学 ›› 2019, Vol. 40 ›› Issue (1): 209-215.doi: 10.7506/spkx1002-6630-20171018-174

β-氨基丁酸处理对采后桃果实还原势的影响及抗病性的诱导作用

陈偲1,2，汪立2，夏明星3，伍冬志2，廖云霞2，汪开拓1,2，郑永华1,*

1.南京农业大学食品科技学院，江苏南京 210095；2.重庆三峡学院生物与食品工程学院，重庆 404100；3.重庆出入境检验检疫局，重庆 404100

出版日期:2019-01-15 发布日期:2019-01-22
基金资助:
国家自然科学基金面上项目（31672209）；重庆市基础与前沿研究计划项目（cstc2015jcyjA80028）；第五批重庆市高校优秀人才支持计划项目（2017019）；重庆三峡学院科研创新团队建设计划项目（201703）；2017年度重庆三峡学院大学生创新性实验计划项目（2017030）

Effect of β-Aminobutyric Acid Treatment on Redox Status and Induction of Disease Resistance in Postharvest Peach Fruits

CHEN Si1,2, WANG Li2, XIA Mingxing3, WU Dongzhi2, LIAO Yunxia2, WANG Kaituo1,2, ZHENG Yonghua1,*

1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China; 3. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 404100, China

Online:2019-01-15 Published:2019-01-22

摘要/Abstract

摘要： 采用10 mmol/L β-氨基丁酸（β-aminobutyric acid，BABA）和病原菌Rhizopus stolonifer处理采后‘白凤’水蜜桃，以此研究BABA处理诱导水蜜桃防卫反应的模式，并从还原势变化角度分析相关诱导抗性机理。结果显示：经10 mmol/L BABA处理的桃果实在20 ℃下贮藏2 d后，伴随着果实的发病，其还原性内源信号分子NO含量以及PpNPR1-like、PpCHI和PpGNS基因表达丰度均明显高于对照组；而BABA＋病原菌处理组PpNPR1-like、PpCHI和PpGNS表达丰度在贮藏前3 d整体上显著高于BABA处理和病原菌接种果实（P＜0.05），在整个贮藏期间3 种基因的表达丰度均处于较高水平。此外，与对照组相比，BABA处理和BABA＋病原菌接种均可显著诱导NO的积累，并同时提升桃果实中磷酸戊糖途径关键酶葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶活力，促使果实中还原型辅酶II（nicotinamide adenine dinucleotide phosphate，NADPH）和还原型谷胱甘肽大量生成，并降低NADP＋和氧化型谷胱甘肽含量，因而有效提高果实组织还原势。通过这些结果可推测，10 mmol/L BABA可通过诱导Priming反应的方式赋予桃果实在受病原菌侵染时更强的抗病性，从而抑制采后软腐病的发生；同时，BABA处理可通过诱导还原性信号分子积累并提高磷酸戊糖途径关键酶活力，进而提升桃果实还原势、活化相关转录因子，以此诱导PRs基因的表达。

关键词: β-氨基丁酸, 桃果实, 还原势, 磷酸戊糖途径, 诱导抗病性

Abstract: The present study was conducted to investigate the pattern of disease resistance induced by 10 mmol/L β-aminobutyric acid (BABA) treatment in postharvest peach (Prunus persica Batsch cv ‘Baifeng’) fruits and to analyze the underlying mechanism with respect to the change in redox status. The results showed that the peach fruits treated with 10 mmol/L BABA alone demonstrated remarkably higher concentrations of the endogenous reductive signaling molecule nitric oxide (NO) and higher expression levels of PpNPR1-like, PpCHI and PpGNS genes after 2 days of storage at 20 ℃ when compared with the control, accompanied by an increase in disease incidence. Meanwhile, the fruits simultaneously treated with BABA and inoculated with the pathogen Rhizopus stolonifer exhibited significantly higher expression levels of PpNPR1-like, PpCHI and PpGNS genes during the first three days of storage than did those receiving either treatment alone (P < 0.05), and maintained high levels throughout the storage period. Moreover, either BABA treatment alone or the combined treatment stimulated the accumulation of NO and simultaneously elevated the activities of glucose 6-phosphatedehydrogenase and 6-phosphogluconate dehydrogenase, which are recognized as key enzymes in the pentose phosphate pathway (PPP), thereby contributing to enhanced production of nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione (GSH) and lower contents of NADP+ and oxidized glutathione and consequently enhanced redox status. Therefore, 10 mmol/L BABA could confer peach fruits with enhanced resistance to R. stolonifera infection by activating the priming defense, thereby reducing the incidence of soft rot disease. At the same time, BABA treatment could induce the accumulation of the reductive signaling molecule and increase the activities of the key enzymes in PPP and consequently enhance the reduction potential and activate relevant transcription factors, thereby inducing the expression of PR gene.

Key words: β-aminobutyric acid, peach fruit, redox status, pentose phosphate pathway, induction of disease resistance

中图分类号:

TS255.36

陈偲，汪立，夏明星，伍冬志，廖云霞，汪开拓，郑永华. β-氨基丁酸处理对采后桃果实还原势的影响及抗病性的诱导作用[J]. 食品科学, 2019, 40(1): 209-215.

CHEN Si, WANG Li, XIA Mingxing, WU Dongzhi, LIAO Yunxia, WANG Kaituo, ZHENG Yonghua. Effect of β-Aminobutyric Acid Treatment on Redox Status and Induction of Disease Resistance in Postharvest Peach Fruits[J]. FOOD SCIENCE, 2019, 40(1): 209-215.

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β-氨基丁酸处理对采后桃果实还原势的影响及抗病性的诱导作用

Effect of β-Aminobutyric Acid Treatment on Redox Status and Induction of Disease Resistance in Postharvest Peach Fruits

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