食品科学 ›› 2010, Vol. 31 ›› Issue (20): 61-66.doi: 10.7506/spkx1002-6630-201020013

• 工艺技术 • 上一篇    下一篇

东海海参多糖酶解提取工艺优化

徐 逯,叶立斌,于 平,励建荣*   

  1. 浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室
  • 收稿日期:2010-01-11 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 励建荣 E-mail:lijianrong@zjgsu.edu.cn

Optimization of Enzymatic Polysaccharide Extraction from Acaudina molpadioides Semper by Response Surface Methodology

XU Lu,YE Li-bin,YU Ping,LI Jian-rong*   

  1. Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology,
    Zhejiang Gongshang University, Hangzhou 310035, China
  • Received:2010-01-11 Online:2010-10-25 Published:2010-12-29
  • Contact: LI Jian-rong* E-mail:lijianrong@zjgsu.edu.cn

摘要:

将现代酶解技术应用于东海海参多糖提取工艺,选取胃蛋白酶、胰蛋白酶、木瓜蛋白酶进行酶解,通过单因素试验确定最佳蛋白酶为胃蛋白酶,并通过响应面分析方法以多糖得率为响应值优化酶解工艺。结果表明,酶解工艺最优条件为胃蛋白酶用量(m/m)0.95%、酶解温度56℃、酶解时间6h、酶解液pH1。在此条件下海参多糖得率为1.65%,该值与响应面模型的预测值1.59% 基本相符。

关键词: 东海海参, 多糖, 蛋白酶, 响应面分析

Abstract:

Papain, pepsin and trypsin were chosen to conduct the single enzyme hydrolysis of Acaudina molpadioides Semper for polysaccharide extraction, and pepsin was the selected enzyme due to the highest polysaccharide yield. The hydrolysis of Acaudina molpadioides Semper with pepsin was optimized by response surface methodology in order to improve polysaccharide yield. The optimal conditions for pepsin hydrolysis were found to be: enzyme load, 0.95%; hydrolysis temperature, 56 ℃; initial pH, 1; and hydrolysis duration, 6 h. An experimental polysaccharide yield of 1.65% was obtained under these conditions, which basically accorded with the model predicted value of 1.59%.

Key words: Acaudina molpadioides Semper, polysaccharide, enzymoysis, response surface methodology

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