食品科学 ›› 2010, Vol. 31 ›› Issue (18): 433-436.doi: 10.7506/spkx1002-6630-201018101

• 技术应用 • 上一篇    下一篇

贝类加工废弃物复合海鲜调味料的制备工艺

陈 超1,2,魏玉西1,刘慧慧1,殷邦忠2,*,赵 玲1,刘 淇2   

  1. 1.青岛大学医学院生物系
    2.中国水产科学研究院黄海水产研究所
  • 收稿日期:2010-01-08 修回日期:2010-06-03 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 殷邦忠 E-mail:yinbz@ysfri.ac.cn
  • 基金资助:

    农业部公益性行业(农业)科研专项(nyhyzx07-047)

Preparation of Compound Seafood Condiments from Shellfish-processed Wastes

CHEN Chao1,2,WEI Yu-xi1,LIU Hui-hui1,YIN Bang-zhong2,*,ZHAO Ling1,LIU Qi2   

  1. 1. Biological Department of Medical College, Qingdao University, Qingdao 266071, China;
    2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  • Received:2010-01-08 Revised:2010-06-03 Online:2010-09-25 Published:2010-12-29
  • Contact: YIN Bang-zhong2,*, E-mail:yinbz@ysfri.ac.cn

摘要:

通过对牡蛎汁、蛤蜊汁蛋白质含量测定及其酶解优化方法探讨,制得牡蛎汁、蛤蜊汁酶解液,并与扇贝裙边酶解液调配出复合调味料。结果表明,牡蛎汁和蛤蜊汁中多肽以及蛋白质含量较高,其酶解优化方案为使用酸性蛋白酶在pH3、60℃酶解5h,牡蛎汁和蛤蜊汁中多肽和蛋白质水解度为0.929。将牡蛎汁、蛤蜊汁酶解液与扇贝裙边酶解液调配制成的复合海鲜调味料,味道鲜美,游离氨基酸态氮含量(15.6mg/mL)高于市售同类产品(13.0mg/mL),且其中甘氨酸、天冬氨酸、丙氨酸、谷氨酸、苏氨酸、丝氨酸和脯氨酸7 种重要呈味氨基酸含量约占总游离氨基酸的50%。

关键词: 蛤蜊汁, 牡蛎汁, 扇贝裙边, 酶解, 水解度, 复合海鲜调味料

Abstract:

Based on the determination of protein content in juice of oysters and crams, and the optimization of enzymatic hydrolysis conditions for protein, enzymatic hydrolysis product of EHL1 was obtained. EHL1 was mixed with enzymatic hydrolysis liquid from scallop mantle (EHL2) to prepare compound seafood condiments. Results indicated that the juice of oysters and crams exhibited higher contents of polypeptides and proteins so that it was necessary for hydrolyzing them to obtain free amino acids as the most important components for sauce with delicious taste. The optimal conditions for enzymatic hydrolysis were hydrolysis at pH 3 and 60 ℃ for 5 h by acidic protease. The degree of hydrolysis was up to 0.929 at this optimal condition. The compound condiment was very delicious due to higher content of free amino acid nitrogen (15.6 mg/mL), which was higher than similar commodity (13.0 mg/mL) in market. Moreover, seven flavor amino acids including Gly, Asp, Ala, Glu, Thr, Ser and Pro were up to 50% of total free amino acids. Therefore, this investigation provided a new idea and method for comprehensive utilization of marine shellfish processing wastes.

Key words: clam juice, oyster juice, scallop mantle, enzymatic hydrolysis, degree of hydrolysis, compound seafood condiment

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