杜仲绿原酸的分离纯化及结构鉴定

doi:10.7506/spkx1002-6630-201014063

食品科学 ›› 2010, Vol. 31 ›› Issue (14): 275-279.doi: 10.7506/spkx1002-6630-201014063

杜仲绿原酸的分离纯化及结构鉴定

卢琪，段家彩，高丽，陈若雯，傅虹飞，潘思轶*

华中农业大学食品科学技术学院

收稿日期:2009-10-19 出版日期:2010-07-15 发布日期:2010-12-29
通讯作者: 潘思轶 E-mail:pansiyi@mail.huau.edu.cn

Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

LU Qi，DUAN Jia-cai，GAO Li，CHEN Ruo-wen，FU Hong-fei，PAN Si-yi*

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2009-10-19 Online:2010-07-15 Published:2010-12-29
Contact: PAN Si-yi* E-mail:pansiyi@mail.huau.edu.cn

摘要/Abstract

摘要：

筛选大孔树脂纯化澄清后的杜仲绿原酸粗提液，并对纯化后的绿原酸进行初步的结构鉴定。采用壳聚糖、活性炭澄清绿原酸的粗提液，选取7 种不同类型的大孔树脂对杜仲绿原酸进行静态吸附解吸实验比较。结果表明，NKA-2 型大孔树脂对绿原酸有较好的选择性，其吸附率和解吸率分别为99.67% 和62.8%。盐酸调整洗脱液的pH 值为3，洗脱液的体积分数为30% 时，洗脱效果最好。澄清后的绿原酸粗提液经乙酸乙酯萃取除去黄酮，再用NKA-2 型树脂进行梯度洗脱，可得到纯度为76.3% 的绿原酸。HPLC-MS 分析表明，绿原酸是咖啡酸和奎尼酸缩合的缩酚酸。

关键词: 绿原酸, 大孔树脂, 纯化, 鉴定

Abstract:

Eucommia ulmoides leaves were extracted with 60% aqueous ethanol under the assistance of ultrasound. The crude extract was then condensed on a rotation evaporator, added with chitosan acetic acid solution and stirred, allowed to stand at 60℃ for 60 min for precipitate sedimentation, and filtrated. The filtrate was decolorized with activated carbon prior to 5-time ethyl acetate extraction to remove flavonoids. The lower phase was collected and a chlorpgenic acid extract was obtained. Seven types of moroporous resins were used to purify the chlorpgenic acid extract, and NKA-2 was the selected resin because it exhibited good selectivity to chlorpgenic acid and the static adsorption and desorption ratios were 99.67% and 62.8%. The use of 30% acidified ethanol (pH 3) provided an optimum elution. After NKA-2 purification in the gradient elution mode, a chlorpgenic acid product with 76.3% purity was obtained. Chlorpgenic acid was confirmed by HPLC-MS analysis to be a condensation product of caffeic acid and quinic acid.

Key words: chlorogenic acid, macroporous resin, purification, identification

中图分类号:

卢琪，段家彩，高丽，陈若雯，傅虹飞，潘思轶*. 杜仲绿原酸的分离纯化及结构鉴定[J]. 食品科学, 2010, 31(14): 275-279.

LU Qi，DUAN Jia-cai，GAO Li，CHEN Ruo-wen，FU Hong-fei，PAN Si-yi*. Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves[J]. FOOD SCIENCE, 2010, 31(14): 275-279.

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杜仲绿原酸的分离纯化及结构鉴定

Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

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