食品科学 ›› 2010, Vol. 31 ›› Issue (12): 110-113.doi: 10.7506/spkx1002-6630-201012025

• 工艺技术 • 上一篇    下一篇

菜芙蓉籽油的微波提取及清除自由基能力研究

仇燕   

  1. 河北科技大学生物科学与工程学院
  • 收稿日期:2009-10-11 修回日期:2010-01-22 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 仇燕 E-mail:qiuyan77@yahoo.com.cn
  • 基金资助:

    河北科技大学校立基金项目(XL200762)

Microwave Extraction and Free Radical Scavenging Activity of Ablmoschus manihot (L.) Medic Seed Oil

QIU Yan   

  1. College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Received:2009-10-11 Revised:2010-01-22 Online:2010-06-15 Published:2010-12-29
  • Contact: QIU Yan E-mail:qiuyan77@yahoo.com.cn

摘要:

采用微波提取法对菜芙蓉籽油进行提取,通过单因素及正交试验确定最佳提取条件。结果表明,采用乙酸乙酯作为萃取溶剂、微波功率540W、液料比10:1(mL/g)条件下提取3min,菜芙蓉籽油出油率最高为25.20%。利用清除羟自由基和超氧阴离子自由基能力作为指标测定菜芙蓉籽油的抗氧化活性,结果表明菜芙蓉籽油具有较好清除羟自由基的能力,EC50 值为0.129mg/mL,抗氧化作用明显。

关键词: 菜芙蓉籽油, 微波提取, 自由基

Abstract:

Ablmoschus manihot (L.) Medic seed oil was extracted by microwave extraction. Through the optimization by single factor and orthogonal array design methods, the optimum conditions for Ablmoschus manihot (L.) Medic seed oil extraction were determined to be: extraction solvent, ethyl acetate; microwave power, 540 W; and liquid/solid ratio, 10:1 for an extraction duration of 3 min. Under these conditions, the maximum oil extraction yield was 25.2%. The abilities of Ablmoschus manihot (L.) Medic seed oil to scavenge hydroxyl and superoxide anion free radicals were both determined, and it presented strong hydroxyl free radical scavenging activity, with a median effective concentration (EC50) of 0.129 mg/mL. So, Ablmoschus manihot (L.) Medic seed oil appears to be a promising free radical scavenger.

Key words: Ablmoschus manihot (L.) Medic seed oil, microwave extraction, free radicals

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