关键词: 沙棘籽粕, 生物碱, 5,11- 二羟基色胺, 心肌细胞, 缺血/ 再灌注

Abstract:

Objective: To investigate the protective effect of alkaloid from seabuckthorn seeds on injured cardiomyocytes in rats. Methods: EtOH extract from seabuckthorn seeds was purified by column chromatography and its structure was elucidated by MS and NMR spectroscopic analyses. A model of cardiac ischemic/reperfusion (I/R) in vitro was established using the primary cultured rat cardiomyocytes. The viability of cardiomyocytes was evaluated by MTT assay and the contents of lactate dehydrogenase (LDH) and creatine kinase (CK) were measured to verify the protective effect on injured rat cardiomyocytes by I/R. Results: The structure of water-soluble alkaloid was identified as 5,11-dihydroxyltryptamine, which could promote the survival rate of cardiomyocytes subjected to I/R injury and reduced the release of myocardial enzymes induced by I/R injury. Conclusion: The identified 5,11-dihydroxyltryptamine from seabuckthorn seeds exhibited an excellent protective effect on cultured neonatal rat cardiomyocytes injured by ischemia/reperfusion at the optimal concentration of 150 mg/L.

Key words: seabuckthorn seed, alkaloid, 5,11-dihydroxyltryptamine, cardiomyocyte, ischemia/reperfusion