食品科学 ›› 2018, Vol. 39 ›› Issue (19): 19-23.doi: 10.7506/spkx1002-6630-201819004

• 基础研究 • 上一篇    下一篇

药桑叶中活性物质的提取及对α-葡萄糖苷酶的抑制作用

郝蒙蒙,崔汉钊,韩爱芝,杨 玲*   

  1. 塔里木大学生命科学学院,塔里木盆地生物资源保护利用兵团重点实验室,新疆 阿拉尔 843300
  • 出版日期:2018-10-15 发布日期:2018-10-24
  • 基金资助:
    国家自然科学基金地区科学基金项目(31460080)

Extraction of Active Substances from Leaves of Morus nigra L. and Evaluation of Their Inhibitory Effect on α-Glycosidase

HAO Mengmeng, CUI Hanzhao, HAN Aizhi, YANG Ling*   

  1. Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Sciences, Tarim University, Alar 843300, China
  • Online:2018-10-15 Published:2018-10-24

摘要: 以药桑叶为实验原料,通过集成提取工艺,获得3 种含量稳定的粗提物成分,研究其对α-葡萄糖苷酶的抑制作用,并进行不同粗提物间组合物对α-葡萄糖苷酶抑制活性的研究。采用乙醇提取、柱色谱分离纯化等方法,获得桑叶中具有潜在降血糖活性的黄酮、多糖和生物碱粗提物;利用α-葡萄糖苷酶抑制模型评价3 种粗提物对α-葡萄糖苷酶的抑制作用,并进一步采用CompuSyn软件对不同粗提物间的相互作用进行统计学分析。结果表明:桑叶中黄酮、生物碱、多糖粗提物对α-葡萄糖苷酶均具有抑制活性,且生物碱粗提物的抑制活性显著高于阳性对照(阿卡波糖),多糖粗提物在高质量浓度作用下具有α-葡萄糖苷酶抑制作用,表现为抑制作用与多糖质量浓度成正比;在本实验所使用的质量浓度与剂量配比内3 种粗提物相互组合时,黄酮+生物碱组合、多糖+生物碱组合表现为拮抗作用;黄酮+多糖组合、黄酮+多糖+生物碱组合在高质量浓度下对抑制α-葡萄糖苷酶表现为协同作用。

关键词: 药桑叶, 黄酮, 生物碱, 多糖, α-葡萄糖苷酶, 协同、拮抗作用

Abstract: In the present study, crude polysaccharides, flavonoids and alkaloids with potential antidiabetic activity were synchronously extracted from the leaves of Morus nigra L. and separated by column chromatography. The α-glycosidase inhibitory activity of the three active substances was investigated individually and in combination. Statistical analysis of the interactions among these substances was carried out using CompuSyn software. The results showed that all three active components from the leaves of Morus nigra L. had α-glucosidase inhibitory activity. Moreover, the alkaloids had better α-glucosidase inhibitory activity than acarbose, while the polysaccharides only at higher concentrations had an obvious inhibitory effect on α-glycosidase, which was directly proportional to concentration. At concentrations tested, the alkaloids showed antagonism against the flavonoids and polysaccharides when used in combination, whereas the flavonoids could synergize with the polysaccharides alone and its mixture with the alkaloids.

Key words: Morus nigra L., flavonoids, alkaloids, polysaccharides, α-glucosidase, synergism and antagonism

中图分类号: