桑叶生物碱对高脂饮食诱导小鼠肝损伤的改善作用及机理

doi:10.7506/spkx1002-6630-20180910-096

食品科学 ›› 2019, Vol. 40 ›› Issue (19): 210-216.doi: 10.7506/spkx1002-6630-20180910-096

桑叶生物碱对高脂饮食诱导小鼠肝损伤的改善作用及机理

王祖文，杨忠敏，杨敏，黄先智，丁晓雯

（1.西南大学食品科学学院，重庆市农产品加工及贮藏重点实验室，食品科学与工程国家级实验教学示范中心，重庆 400716；2.西南大学科技处，重庆 400716）

出版日期:2019-10-15 发布日期:2019-10-25
基金资助:
现代农业产业技术体系建设专项（CARS-18）

Effect and Mechanism of Mulberry Leaf Alkaloids on the Improvement of High-Fat Diet Induced Liver Injury in Mice

WANG Zuwen, YANG Zhongmin, YANG Min, HUANG Xianzhi, DING Xiaowen

(1. Key Laboratory of Processing and Storage of Agricultural Products of Chongqing, National Demonstration Center for Experimental Food Science and Technology Education, College of Food Science, Southwest University, Chongqing 400716, China; 2. Department of Science and Technology, Southwest University, Chongqing 400716, China)

Online:2019-10-15 Published:2019-10-25

摘要/Abstract

摘要： 目的：探讨桑叶生物碱对高脂饮食诱导小鼠肝损伤的改善作用与机理，为桑叶的科学利用提供参考。方法：用高脂饲料喂饲小鼠的同时灌胃不同剂量的桑叶生物碱，于第4、8、12、16周末检测小鼠肝脏系数和血浆谷丙转氨酶（alanine aminotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST）活力；取肝组织进行苏木精-伊红染色观察组织病理学改变；实时定量聚合酶链式反应测定肝组织炎症因子白细胞介素-10（interleukin-10，IL-10）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和细胞凋亡因子Bax、Bcl-2 mRNA表达水平。结果：与高脂对照组比，随着桑叶生物碱灌胃剂量的增加和时间的延长，小鼠肝脏系数及ALT、AST活力显著下降（P＜0.05），肝脏中脂质沉积和脂肪变性得到改善，IL-10、Bcl-2 mRNA表达水平显著上升（P＜0.05），TNF-α、Bax mRNA表达水平显著降低（P＜0.05）。200 mg/kg mb桑叶生物碱灌胃16 周能使肝损伤小鼠机体各指标恢复到正常水平。结论：桑叶生物碱对高脂饮食导致的肝损伤有良好的改善作用，其机制可能与调节炎症因子（TNF-α、IL-10）和细胞凋亡因子（Bax、Bcl-2）的mRNA表达水平有关。

关键词: 桑叶生物碱, 高脂饮食, 肝损伤, 炎症因子, 凋亡因子

Abstract: Objective: Our aim was to study the effect and underlying mechanism of mulberry leaf alkaloids on the improvement of liver injury induced by a high-fat diet in mice for the purpose of providing a basis for the reasonable use of mulberry leaves. Methods: Mice were fed with a high-fat diet and administered by gavage with three doses of mulberry leaf alkaloids for 16 weeks. After 4, 8, 12 and 16 weeks of administration, liver index and the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in plasma were detected, histopathological characteristics of liver tissue were observed by hematoxylin and eosin staining and the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-10, Bax and Bcl-2 mRNA in liver tissue was also detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Results: Compared with the high-fat diet control group, liver index and plasma ALT and AST activity were decreased significantly (P < 0.05), lipid deposition and steatosis in the liver were ameliorated, the mRNA expression levels of IL-10 and Bcl-2 were increased significantly (P < 0.05), and the mRNA expression levels of TNF-α and Bax were decreased significantly by administration of the alkaloids in a dose- and time-dependent manner (P < 0.05). The indexes altered by liver injury returned to normal after administration of the alkaloids at a dose of 200 mg/kg mb for 16 weeks. Conclusion: Mulberry leaf alkaloids significantly improve high-fat-induced liver injury in mice and its mechanism may be related to the regulated mRNA expression of TNF-α, IL-10, Bcl-2 and Bax.

Key words: mulberry leaf alkaloids, high-fat diet, liver injury, inflammatory factor, apoptotic factor

中图分类号:

R285.5
TS218

王祖文，杨忠敏，杨敏，黄先智，丁晓雯. 桑叶生物碱对高脂饮食诱导小鼠肝损伤的改善作用及机理[J]. 食品科学, 2019, 40(19): 210-216.

WANG Zuwen, YANG Zhongmin, YANG Min, HUANG Xianzhi, DING Xiaowen. Effect and Mechanism of Mulberry Leaf Alkaloids on the Improvement of High-Fat Diet Induced Liver Injury in Mice[J]. FOOD SCIENCE, 2019, 40(19): 210-216.

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桑叶生物碱对高脂饮食诱导小鼠肝损伤的改善作用及机理

Effect and Mechanism of Mulberry Leaf Alkaloids on the Improvement of High-Fat Diet Induced Liver Injury in Mice

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