Optimization of Lnulinase Fermentation by Aspergillus niger X-6 Using Response Surface Methodology

doi:10.7506/spkx1002-6630-201023032

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 138-141.doi: 10.7506/spkx1002-6630-201023032

• Bioengineering • Previous Articles Next Articles

Optimization of Lnulinase Fermentation by Aspergillus niger X-6 Using Response Surface Methodology

LUO Deng-lin，YUAN Hai-li，ZENG Xiao-yu，LIU Jian-xue

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China

Received:2010-03-31 Revised:2010-10-22 Online:2010-12-15 Published:2010-12-29
Contact: LUO Deng-lin E-mail:luodenglin@sohu.com

Abstract

Abstract:

The objective of this study was to obtain the optimum conditions for Aspergillus niger X-6 fermentation to improve inulinase yield. The effects of the amounts of wheat bran, inulin, peptone and yeast extract in the fermentation medium, fermentation time and temperature, pH, and inoculum size on inulinase yield were evaluated using Plackett- Burmen design. Inulinase yield was significantly influenced by the amount of wheat bran addition, fermentation time and pH. Subsequently, the optimization of the three factors was conducted using Box-Behnken experimental design and response surface methodology, in which, a quadratic polynomial model for inulinase yield was established. The optimal fermentation conditions for inulinase prodtion were determined to be: the amount of wheat bran addition, 4.64%; fermentation time, 81.5 h; and initial mdedim pH 6.0. Under these conditions, the yield of inulinase reached 20.42 U/mL, 30.81% higher than that before optimization.

Key words: inulinase, Aspergillus niger X-6, fermentation, response surface methodology

CLC Number:

TS201.2

LUO Deng-lin，YUAN Hai-li，ZENG Xiao-yu，LIU Jian-xue. Optimization of Lnulinase Fermentation by Aspergillus niger X-6 Using Response Surface Methodology[J]. FOOD SCIENCE, 2010, 31(23): 138-141.

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Optimization of Lnulinase Fermentation by Aspergillus niger X-6 Using Response Surface Methodology

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