FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (12): 267-272.doi: 10.7506/spkx1002-6630-20190319-239

• Safety Detection • Previous Articles     Next Articles

Fabrication of Non-enzymatic Amperometric Glucose Biosensor Based on Fe3O4-Poly-(γ-glutamic acid)@Au

GUAN Huanan, GONG Dezhuang, SONG Yan, LIU Bo, HAN Bolin, YANG Fan, CUI Linlin, ZHANG Na   

  1. (College of Food Engineering, Harbin University of Commerce, Harbin 150076, China)
  • Online:2020-06-25 Published:2020-06-22

Abstract: Herein we report a novel highly sensitive and selective non-enzymatic glucose biosensor fabricated using a Fe3O4-poly-(γ-glutamic acid)@Au (Fe3O4-PGA@Au)-modified glassy carbon electrode. By a green reduction and adsorption method, Fe3O4-PGA@Au was successfully prepared and characterized by X-ray differentiation (XRD), Fourier transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). The reaction temperature, pH value, scan rate and time were optimized. Cyclic voltammetry was used to investigate the biosensor performance. The results showed that Fe3O4-PGA@Au had good electrocatalysis performance. Good linearity was observed between the peak current density of the biosensor and glucose concentration in the range of 0.1–5.0 and 10–250 μmol/L with a detection limit (LOD) of 0.74 μmol/L (RSN = 3). In addition, the biosensor showed excellent reproducibility. It could efficiently resist the interference of coexistent substances.

Key words: glucose, amperometric biosensor, nanozymes, gold nanoparticles, Fe3O4, poly-(γ-glutamic acid)

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