Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester

FOOD SCIENCE ›› 0, Vol. ›› Issue (): 59-63.

Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester

Received:2010-09-03 Revised:2011-01-05 Online:2011-02-25 Published:2011-01-20

Abstract

Abstract: Transesterification was conducted in soybean oil with phytosterol under the catalysis of Novozym 435 lipase. The optimum reaction conditions were obtained by single factor experiments and response surface analysis to be reaction between soybean oil and added phytosterol at a concentration of 5.2% for 3 h at 101 ℃ in the presence of 1% lipase. The rate of transesterification was 85.67% under these conditions.

Key words: phytosterol, soybean oil, transesterification, lipase

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Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester

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