A Comparative Investigation into Deproteinization Methods for Silkworm Pupa Polysaccharides

Abstract

Abstract: Protein removal rate and polysaccharide loss rate were used to evaluate and compare the effectiveness of Savag, s method, trichloroacetic acid (TCA) method, papain method and papain-TCA (papain hydrolysis followed by TCA precipitation) method in deproteinizing silkworm pupa polysaccharides. Papain-TCA method was found to be the best method for the deproteinization of silkworm pupa polysaccharides, and the optimal operating conditions were 1.5 h hydrolysis with papain at an enzyme-to-substrate of 2.0% and 50 ℃ followed by once treatment with TCA. A protein removal rate of 97.98% and a polysaccharide loss rate of 18.76% were achieved under such conditions. Only one absorption peak was observed in the UV-visible scanning spectrum of deporteinized silkworm pupa polysaccharides, indicating the removal of almost all proteins.

Key words: pupa, polysaccharide, protein, removal

CLC Number:

R284.2

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A Comparative Investigation into Deproteinization Methods for Silkworm Pupa Polysaccharides

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