Abstract: Objective: To explore the effect of lactosucrose on hime metabolites in established rat model with ulcerative colitis. Methods: TNBS was used to induce ulcerative colitis in rats, and the obtained ulcerative colitis rats were randomly divided into 4 groups including the normal group, model control (MC) group, lactosucrose (LS) group and sulfasalazine (SASP) group. The last two groups were orally administered 2 mL of normal saline containing 250 mg/kg LS and normal saline containing 250 mg/kg SASP for 21 consecutive days, respectively. The normal and model groups were given the same volume of normal saline. All the mice were killed after 24 hours of fasting after the last administration. The short chain fatty acid contents, pH and NH3-N in cecum and colon were determined. Results: Compared with the NC group, a significantly decreased acetic acid and butyric acid in cecum and colon, a significantly declined propionic acid in colon and a significantly increased pH and NH3-N were observed in MC group (P ＜ 0.01); Compared with MC, when the rats treated with LS, acetic acid (P ＜ 0.05) and butyric acid (P ＜ 0.01) in cecum were significantly increased; pH (P＜0.05) and NH3-N (P ＜ 0.01) were significantly decreased; NH3-N in colon were significantly decreased (P ＜ 0.05). Conclusion: LS can be used as a functional food with potential therapeutic effect on colitis. The mechanism for relieving the injury of colon tissues in colitis may be due to the increased content of butyric acid and decreased content of NH3-N in the intestinal tract.

Key words: lactosucrose, colitis, metabolism, short chain fatty acid, NH3-N