Optimization of Operating Conditions for HPLC Determination of Biogenic Amines

Abstract

Abstract: In order to establish a reversed-phase HPLC method for the determination of 8 biogenic amines, mobile phase composition, elution mode, detector type and detection wavelength were optimized. Gradient elution using a mobile phase composed of acetonitrile, ammonium acetate and ultra-pure water proved optimal, and 8 biogenic amines were completely separated within 30 min, showing symmetrical peak shapes without tails and extension. The optimization resulted in a time-saving analytical method. Moreover, fluorescence detector (FLD) presented higher selectivity and sensitivity when compared with diode array detector (DAD) so that it can meet the requirements for accurate determination of biogenic amines.

Key words: high performance liquid chromatography (HPLC), biogenic amines, measurement conditions optimization

CLC Number:

TS207.5

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