Biofilm-forming Capabilities of Lactobacillus bulgaricus and Streptococcus thermophilus

Abstract

Abstract: The biofilm-forming capabilities of Streptococcus thermophilus and Lactobacillus bulgaricus on different carriers (coconut, stainless steel mesh, plastic sheet, glass sheet or ceramic parts) was studied by using placed slice and plate culture count methods. The results showed that stainless steel mesh and coconut were suitable carriers for biofilm formation. During 7 days, the CFU (colony-forming units) of the single-species biofilm on coconut and stainless steel mesh initially reached 1 × 107 CFU/cm2 and then was kept at 3.2 × 106 CFU/cm2. The mixed-species biofilm was stable at approximately 1 × 107 CFU/cm2 in continuous seven days. In addition, scanning electron microscope was used to observe mixed-species biofilm formed by S. thermophilus and L. bulgaricus, and a typical mixed-species biofilm was observed on coconut and stainless steel mesh.

Key words: lactic acid bacteria, biofilm, carrier, stability

CLC Number:

TS201.3

References

[1] HALL-STOODLEY L, COSTERTON J W, STOODLEY P. Bacterial biofilms: from the natural environment to infectious diseases[J]. Nature Reviews Microbiology, 2004, 2(2): 95-108.
[2] 李彤, 庄辉. 细菌生物膜的研究进展[J]. 中华微生物学和免疫学杂志, 2002, 22(3): 343-346.
[3] 徐蓉蓉. 生物膜中细菌的代谢特点及影响因素[J]. 国际口腔医学杂志, 2004, 31( 1): 42-43.
[4] DONLAN R M, COSTERTON J W. Biofilms: survival mechanisms of clinically relevant microorganisms[ J]. Clinical Microbiology Reviews, 2002, 15(2): 167-193.
[5] BALABAN N. Control of Biofilm Infections by Signal Manipulation[M]. Newyork: Springer-Verlag, 2008, 2: 157-170.
[6] SPERANZA B, SINIGAGLIA M, CORBO M R. Non starter lactic acid bacteria biofilms: A means to control the growth of Listeria monocytogenes in soft cheese[J]. Food Control, 2009, 20: 1063-1067.
[7] 张川. 光合细菌生物膜制氢反应器传输与产氢特性[D].重庆: 重庆大学, 2010.
[8] LOO C Y, CORLISS D A, GANESHKUMAR N. Streptococcus gordonii Biofilm Formation: Identification of Genes that Code for Biofilm Phenotypes[J]. Journal of Bacteriology, 2000, 182(5): 1374-1382.
[9] O’TOOLE G A, KOLTER R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis[J]. Molecular Microbiol, 1998, 28: 449-461.
[10] 周德庆. 微生物学实验教程[M]. 2版, 北京: 高等教育出版社, 2005.
[11] 李京宝, 韩峰, 于文功. 细菌生物膜研究技术[J]. 微生物学报, 2007, 47 (3) : 558-561.
[12] 沈萍, 范秀荣, 李广武.微生物学实验[M]. 3版, 北京: 高等教育出版社, 2006.
[13] RANGASWAMY V, RAMAKRISHNA S V. Lactic acid production by Lactobacillus delbrueckii in a dual reactor system using packed bed biofilm reactor[J]. Applied Microbiology, 2008, 46: 661-666.
[14] KUBOTA H, SENDA S, TOKUDA H , et al. Stress resistance of biofilm and planktonic Lactobacillus plantarum subsp.plantarum JCM 1149[J]. Food Microbiology, 2009, 26: 592-597.

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