Separation and Purification of Naringin by Solid Phase Extraction Using Strong Anion Exchange Cartridge

Abstract

Abstract: Purpose: A solid-phase extraction procedure for the separation and purification of naringin (NG) was developed using strong anion exchange (SAX) cartridge. Method: Naringin was adsorbed onto the cartridge by electrostatic interaction between naringin and functional groups. Several variables affecting the elution performance of SAX, such as ionic strength of elution, elution volume and elution rate, were studied. Results: The application of 5 mL of sodium bicarbonate solution (0.1 mol/L, pH = 9.0) to elute SAX cartridge at a flow rate of 0.5 mL/min resulted in a recovery rate of 91%. Conclusions: The established method is simple and efficient.

Key words: solid phase extraction, strong anion exchange (SAX), naringin

CLC Number:

References

[1]贾冬英, 姚开, 谭敏, 等.柚果皮中生理活性成分研究进展[J].食品与发酵工业,2001,27(11):74-78
[2]周强.柚皮与柚籽中果胶、柚皮苷和类柠檬苦素的提取研究[D]. 长沙: 湖南大学; 2007
[3]JEON S M, PARK Y B, CHOI M S.Antihypercholesterolemic property of naringin alters plasma and t5 lipids, cholesterol-regulating enzymes, fecal sterol and t5 morphology in rabbits[J].Clinical Nutrition,2004,23(5):1025-1034
[4]LEE C H, JEONG T S, CHOI Y K, et al.Anti-atherogenic effect of citrus flavonoids, naringin and naringenin, associated with hepatic ACAT and aortic VCAM-1 and MCP-1 in high cholesterol-fed rabbits[J].Biochemical and Biophysical Research Communications,2001,284(3):681-688
[5]LEE C H, HYUN B H, CHOI Y K, et al.Anti-atherogenic effects of citrus bioflavonoids, naringin and naringenin, and their metabolite in high cholesterol-fed rabbits[J].Arteriosclerosis, Thrombosis, and Vascular Biology,2001,21(4):289-289
[6]FOTSIS T, PEPPER M, ADLERCREUTZ H , et al.Genistein,a dietary ingested isoflavonoid,inhibits cell proliferation and in vitro angiogenesis[J].The Journal of Nutrition,1995,125(3):790-S
[7]AMARO M I, ROCHA J, VILA-REAL H, et al.Anti-inflammatory activity of naringin and the biosynthesised naringenin by naringinase immobilized in microstructured materials in a model of DSS-induced colitis in mice[J].Food Research International,2009,42(8):1010-1017
[8]RIBEIRO M, BARATEIRO A, VILA-REAL H, et al.Anti-inflammatory effect of naringin and naringenin on TNF-α secretion in cultured cortical astrocytes after stimulation with LPS[J].New Biotechnology,2009,25:10-
[9]贾冬英, 姚开, 何强, 等.柚皮苷-金属配合物的抗氧化活性研究[J].中国油脂,2005,30(4):30-33
[10]李赤翎, 俞建, 曾亮, 等.柚皮苷对猪肉的抗氧化作用的研究[J].食品研究与开发,2008,29(10):20-23
[11]CAVIA-SAIZ M, BUSTO M D, PILAR-IZQUIERDO M C, et al.Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: a comparative study[J].Journal of the Science of Food and Agriculture,2010,90(7):1238-1244
[12]JEON S M, BOK S H, JANG M K, et al.Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits[J].Life Sciences,2001,69(24):2855-2866
[13]马波, 李梦龙, 周在德.黄酮体化合物抗肿瘤活性的量子化学研究[J].化学研究与应用,2002,14(2):149-152
[14]MARTIN M J, MARHUENDA E, PEREZ-GUERRERO C, et al.Antiulcer effect of naringin on gastric lesions induced by ethanol in rats[J].Pharmacology,1994,49(3):144-150
[15]GAO S, LI P, YANG H, et al.Antitussive effect of naringin on experimentally induced cough in guinea pigs[J].Planta medica,2011,77(1):16-21
[16]冯宝民, 蒋革, 贾景明, 等.柚皮苷和新橙皮苷抗过敏作用的研究[J].大连大学学报,2005,26(4):63-64
[17]WEI M.nullYANG Z, LI P, et al: Anti-osteoporosis activity of naringin in the retinoic acid-induced osteoporosis model[J].. The American Journal of Chinese Medicine,2007,:-
[18]庞瑞, 杨中林.不同产地不同品种柚皮中总黄酮和柚皮苷的含量比较[J].药学与临床研究,2007,15(3):205-207
[19]魏胜华, 孟娜, 伍和芳, 等.毛细管电泳法测定柚皮中柚皮苷的含量[J].光谱实验室,2011,28(2):853-856
[20]孙家娟, 刘彬, 尚永辉, 等.流动注射双安培法测定柚皮苷[J].分析试验室,2006,25(12):29-29
[21]李晓光, 林励, 陈志霞.薄层扫描法测定化橘红中柚皮苷含量[J].浙江中医学院学报,2003,27(1):82-82